方真华，吴华，马伟明，赵文春.50 Hz电磁场对小鼠骨髓间充质干细胞增殖的影响[J].中华物理医学与康复杂志,2004,(1):
扫码阅读全文
|
| 50 Hz电磁场对小鼠骨髓间充质干细胞增殖的影响 |
|
| |
| DOI： |
| 中文关键词: 脉冲电磁场 间充质干细胞 增殖 |
| 英文关键词: Pulsed electromagnetic fields Mesenchymal stem cells Proliferation |
| 基金项目: |
|
| 摘要点击次数: 1739 |
| 全文下载次数: 1655 |
| 中文摘要: |
| 目的探讨小鼠骨髓间充质干细胞经脉冲电磁场辐射不同时间后其细胞增殖水平的变化。 方法用密度梯度离心法分离小鼠骨髓间充质干细胞，再经贴壁筛选法筛选，对生长良好的第3代小鼠骨髓间充质干细胞进行不同时间的脉冲电磁场辐射（频率50 Hz、正弦波形、不同强度），用MTT 法、流式细胞仪法测定细胞生长增殖水平及细胞周期变化。 结果小鼠骨髓间充质干细胞经脉冲电磁场辐射3 d(每天2次，每次30 min)后，各实验组细胞增殖水平均有明显提高，差异有显著性意义(P<0.05)。采用流式细胞仪测定细胞周期，发现各实验组（S+G2/M）期细胞数量较对照组均有不同程度的增长， 差异有显著性意义(P<0.05)。各组均未发现DNA倍体异常细胞。 结论小鼠骨髓间充质干细胞经频率为50 Hz、正弦波形、强度分别为4 mT，3 mT，2 mT，1 mT的脉冲电磁场辐射3 d（每天2次，每次 30 min，间隔12 h）后，能明显促进该细胞体外培养的增殖水平。 |
| 英文摘要: |
| Objective To study the effect of pulsed electromagnetic fields (PEMFs) on the proliferation of bone mesenchymal stem cells in mice. MethodsThe bone marrow mesenchymal stem cells were obtained by using gradient centrifuge method， and then selected by the adhesive method. The third generation cells were irradiated by use of pulsed electromagnetic fields of 50Hz，sinusoid wave， at different intensities and for different time. The method of MTT was employed to evaluate the level of proliferation. The parameters regarding the variation of the cell cycle were detected with the flow cytometry (FCM). ResultsThe proliferation of the bone mesenchymal stem cells in mice was greatly accelerated after 3 days of irradiation by pulsed electromagnetic fields of 50Hz，sinusoid wave (twice daily， 30 min each time ) at the intensities of 4mT，3mT，2mT and 1mT. The percentage of cells at the (S+G2/M) phase was increased significantly (P<0.05). No abnormal ploidy was found in every group. ConclusionThree days of irradiation (twice daily， 30 min each time) of pulsed electromagnetic fields of 50Hz，sinusoid wave at the intensities of 4mT， 3mT， 2mT and 1mT could increase the proliferation of bone mesenchymal stem cells of mice in vitro. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
