邓晓红，张苏明，许予明，许康，喻志源.短暂缺氧、血清剥夺再灌流后神经细胞顿抑现象的研究[J].中华物理医学与康复杂志,2004,(3):
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| 短暂缺氧、血清剥夺再灌流后神经细胞顿抑现象的研究 |
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| DOI： |
| 中文关键词: 缺氧 血清剥夺 再灌流 神经细胞顿抑 能量代谢 |
| 英文关键词: Anoxia Serum deprivation Reperfusion Nerve cells stunning Energy metabolism |
| 基金项目:国家自然科学基金资助项目（No.30070825） |
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| 摘要点击次数: 981 |
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| 中文摘要: |
| 目的研究短暂缺氧、血清剥夺复氧复注血清（再灌流）后有无神经细胞顿抑现象存在及可能的发生机制。 方法将PC12细胞随机分为正常对照组和缺氧组，每组根据不同的再灌流时间点又分为3个亚组（缺氧组分别为缺氧15 min再灌流1 h组、3 h组、6 h组，正常对照组各亚组的时间点与缺氧组相对应）。测定短暂缺氧血清剥夺再灌流后不同时间点的三磷酸腺苷含量、线粒体膜电位和细胞活性。 结果与正常对照组相比，缺氧血清剥夺15 min再灌流1 h后的三磷酸腺苷含量、线粒体膜电位、细胞活性显著降低，差异具有显著性意义（P<0.05）； 缺氧血清剥夺15 min再灌流6 h后基本恢复至正常水平，差异无显著性意义（P>0.05）。 结论短暂缺氧血清剥夺再灌流后，存在低能量状态，且可完全恢复，表明可能存在神经细胞顿抑现象，其原因可能与缺氧血清剥夺再灌流后线粒体呼吸链上的琥珀酸脱氢酶等活性下降及线粒体的膜电位变化等有关。 |
| 英文摘要: |
| Objective To identify whether there is nerve cells stunning after transient anoxia-serum deprivation / reperfusion and to explore the probable underlying mechanism of the phenomenon. MethodsThe PC12 cells were randomly divided into a control group and a anoxia group， the two groups were divided into 3 subgroups, respectively, according to time of reperfusion after 15 minutes of anoxia-serum deprivation. The content of adenosine triphosphate, mitochondria membrane potential and cell activity were determined at different time points after transient anoxia-serum deprivation / reperfusion. ResultsThe content of adenosine triphosphate , mitochondria membrane potential and cell activity after 15 minutes of anoxia-serum deprivation plus 1 hour of reperfusion in the anoxia group were significantly lower than those in the control group（P<0.05）and they recovered to the level of the control group after 6 hours of reperfusion. ConclusionThere was lower energy status after transient anoxia-serum deprivation / reperfusion, the lower energy status can recover to the normal level. The result showed that there might be nerve cells stunning. This phenomenon probably resulted from lower succinate dehydrogenase activity in the respiratory chain of the cells and the changes of mitochondria membrane potential. |
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