常燕群，刘勇，范华燕.血管内皮生长因子165对局灶性脑梗死的影响[J].中华物理医学与康复杂志,2004,(1):
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| 血管内皮生长因子165对局灶性脑梗死的影响 |
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| DOI： |
| 中文关键词: 脑梗死 血管内皮生长因子165 基因治疗 |
| 英文关键词: Cerebral infarct Vascular endothelial growth factor165 Gene therapy |
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| 中文摘要: |
| 目的探讨血管内皮生长因子165(VEGF165)基因对大鼠脑梗死的治疗作用。 方法用线栓法制成Wistar大鼠持续性大脑中动脉闭塞（MCAO）模型，将质粒载体与VEGF165基因构成的重组DNA(puCCAGGS/hVEGF165)经颅骨孔注入到梗死区。术后第8天断头取脑，经2%红四氮唑染色后测定各组脑梗死体积，采用逆转录PCR和免疫组织化学方法检测VEGF165基因的表达及血管增生情况。 结果治疗组VEGF mRNA、VEGF蛋白表达増高，治疗组脑梗死区周围血管数量为50.76条/高倍镜视野(×400)，明显高于对照组的40.67条/高倍镜视野(×400)，P<0.01；治疗组脑梗死体积为9.32%，明显小于对照组的23.68%，P<0.01。 结论在质粒载体的介导下，VEGF165基因可以转化到缺血脑组织中，并表达VEGF165蛋白，可明显缩小脑梗死体积，治疗脑梗死。 |
| 英文摘要: |
| Objective To study the effects of naked DNA encoding vascular endothelial growth factor165 (VEGF165)on cerebral infarction in rats. MethodsFollowing establishment of a permanent middle cerebral artery occlusion (MACO) model by nylon suture embolization in Wistar rats， the puCCAGGS/hVEGF165 was directly injected into the ischemic tissues through skull hole. Seven days later， the rats were sacrificed. The infarct volume was measured by 2% TTC staining technique， then the expression of VEGF165 gene and vascular proliferation were measured by use of RT-PCR and immumohistochemistry methods. ResultsExpression of VEGF165 mRNA and VEGF protein in the therapy group increased. Compared with the control group， the number of vessels of the therapy group was significantly higher (50.76/HPF vs 40.67/HPF)(P<0.01)， and the infarct volume of therapy group was obviously smaller in the therapy group (9.32% vs 23.68%) (P<0.01). ConclusionTopically applied VEGF165 gene can be transformed into the ischemia tissues in the brain and express VEGF165 protein， which can help promote vascular proliferation and reduce the infarct volume. |
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