刘仁刚，罗贤雯，周洁萍，刘云华，骆清铭.高温对HeLa细胞周期及周期因子Cyclin E和Cyclin A的影响[J].中华物理医学与康复杂志,2004,(12):
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| 高温对HeLa细胞周期及周期因子Cyclin E和Cyclin A的影响 |
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| DOI： |
| 中文关键词: 高温 细胞周期 Cyclin E Cyclin A |
| 英文关键词: Hyperthermia Cell cycle Cyclin E Cyclin A |
| 基金项目:国家自然科学基金重点资助项目（No．59836240） |
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| 摘要点击次数: 1505 |
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| 中文摘要: |
| 目的探讨高温时HeLa细胞周期进程受阻与细胞周期因子Cyclin E和Cyclin A表达的关系。 方法将人子宫颈癌细胞株（HeLa细胞）分为37℃、40℃和43℃ 3个温度组，每组均以相应的温度作用1 h。采用流式细胞仪检测各组HeLa细胞的细胞周期、免疫细胞化学方法测定其Cyclin E和Cyclin A的表达，并以图像分析仪测定免疫组织化学反应物的光密度（OD）。 结果40℃和43℃时，细胞周期受阻，大量细胞滞留于G1期，S期和G2/M期的细胞明显减少。Cyclin E和Cyclin A免疫反应阳性细胞明显减少、反应强度明显减弱。随着温度的升高，Cyclin E和Cyclin A免疫反应产物的平均OD值逐渐减小。 结论高温抑制了Cyclin E和Cyclin A的活性，阻滞了细胞周期的进程，使HeLa细胞滞留于G1期。 |
| 英文摘要: |
| Objective To investigate the relationship between the expression of Cyclins E and A and the cell cycle block caused by hyperthermia of HeLa cells. Methods The HeLa cells were divided into three groups and treated with temperatures of 37℃,40℃ and 43℃, respectively, for 1h. Then the cell cycle phases were measured by use of the flow cytometry, and the expression of Cyclins E and A were assessed by use of immunocytochemistry methods. The optical densities (OD) of immunohistochemical reactants were appraised by use of image analyzer. ResultsThe cell cycle of HeLa cells was blocked at 40℃ and 43℃, and a large number of cells were arrested in G1 phase while the cells in S and G2/M phases were decreased markedly. The positive cells of immunoreaction of Cyclins E and A were decreased significantly and the immunoactivities of Cyclins E and A were weakened significantly. The average ODs of immunoreaction of Cyclins E and A were reduced gradually from 37℃ to 43℃. Conclusion The activities of Cyclins E and A were inhibited by hyperthermia, and the cell cycle of HeLa cells was blocked and arrested in G1 phase. |
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