王艺喆,李婷婷,孙大明,等.机械振动对去卵巢骨折大鼠miR-214-3p、IL-1β的影响[J].中华物理医学与康复杂志,2021,43(10):871-874
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| 机械振动对去卵巢骨折大鼠miR-214-3p、IL-1β的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2021.10.002 |
| 中文关键词: 机械振动 骨质疏松骨折 miR-214-3p 白细胞介素-1β |
| 英文关键词: Mechanical vibration Bone fractures MicroRNA-214-3p Interleukin-1β |
| 基金项目:湖北省自然科学基金资助项目（2011CDB312）;武汉体育学院青年教师科研基金资助项目（2014QZ09） |
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| 中文摘要: |
| 目的 观察机械振动对去卵巢骨折大鼠骨折断端miR-214-3p及血清中白细胞介素-1β（IL-1β）表达的影响。 方法 选取30只3月龄雌性Wistar大鼠，按照随机数字表法将其分为对照组、模型组、振动组，每组10只。振动组大鼠骨折术后5 d进行全身振动治疗，振动频率35 Hz，每日20 min，每周5 d。对照组自然饲养，模型组行卵巢摘除术后自然饲养，均不予振动治疗。术后2周、6周，采用Lane-Sandhu X射线评分系统评价大鼠的骨折愈合质量，采用RT-PCR技术检测大鼠骨中miR-214-3p的含量，用酶联免疫吸附测定法检测大鼠血清中IL-1β的含量。 结果 术后2周、6周，模型组断端骨痂生长评分低于对照组，振动组断端骨痂生长评分高于模型组（P＜0.05）。与对照组同时间点比较，模型组、振动组术后2周、6周骨折断端miR-214-3p含量均较高（P＜0.05）。与模型组同时间点比较，振动组术后6周骨折断端miR-214-3p含量（0.71±0.03）较低（P＜0.05）。模型组大鼠术后2周、6周的IL-1β较对照组高，振动组大鼠术后2周、6周的IL-1β较对照组及模型组低（P＜0.05）。 结论 机械振动可抑制骨折断端miR-214-3p的表达，降低IL-1β水平，加速骨质疏松后骨折愈合过程。 |
| 英文摘要: |
| Objective To observe the effect of mechanical vibration on the expression of microRNA-214-3p and serum interleukin-1β (IL-1β) in the broken ends of fractured bones in ovariectomized rats. Methods Thirty 3-month-old female Wistar rats were randomly divided into a control group, a model group, and a vibration group, each of 10. An operation of ovariotomy was performed in those of the model and vibration groups to establish osteoporosis model. Five months later a model of mid femur fracture was made with animals in all the groups. Five days after their fracture, the vibration group received 20 minutes of whole-body vibration treatment at 35Hz for 5 days a week, while the control group and the model group received natural rearing without any additional intervention At 2 and 6 weeks after the operation, the Lane-Sandhu X-ray scoring system was used to evaluate the quality of fracture healing, and reverse-transcription polymerase chain reactions and enzyme-linked immunosorbent assays were used to detect miR-214-3p in the fractured bones and the serum levels of IL-1β. Results At 2 weeks and 6 weeks after the operation the average growth score of the broken ends in the model group was significantly lower than that of the control group, while that of the vibration group was significantly higher than the model group′s average. Compared with the control group at the same time point, the average miR-214-3p content of the model and vibration groups was significantly higher 2 and 6 weeks after the surgery. Compared with the model group, the average level of miR-214-3p of the fractured ends of the vibration group was significantly lower at 6 weeks. Two and six weeks after the surgery, the average IL-1β of the model group was significantly higher than the control group′s average, and that of the vibration group was significantly lower. Conclusion Mechanical vibration can promote osteoporotic fracture healing by inhibiting the expression of miR-214-3p and reducing the level of IL-1β at the broken ends of fractured bones. |
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