Objective To explore the effect of repetitive transcranial magnetic stimulation (rTMS) on contents of monoamine and amino acid transmitters in rabbits with cerebral hemorrhage and the underlying mechanism. MethodsFifty-four rabbits were divided randomly into 3 groups, group A (receiving rTMS) and group B (no rTMS applied) were the experimental groups and group C was the control group. The cerebral hemorrhage model was made by injection of self-body blood, and the control group was made by injection of saline. The rabbits in group A were treated with rTMS once daily and those in groups B and C without rTMS. Rabbits in all 3 groups were executed separately after 12h, 24h, 72h and 1w since the model were established. High performance liquid chromatography (HPLC) method was used to detect the contents of Glu, Asp, Gly, GABA, DA, NA,A and 5-HT in the perihematomal brain tissues. ResultsCompared with group C, the contents of Glu and Asp significantly increased and Gly and GABA significantly decreased, respectively, in group B (P<0.01).Compared with group B, the contents of Glu and Asp significantly decreased and Gly and GABA significantly increased, respectively, (P<0.01),the contents of DA and 5-HT significantly increased in 12h and 24~72h, respectively (P<0.01), while the changes of NA and A were no significant at different time points in group A. ConclusionrTMS could be beneficial for the treatment of cerebral hemorrhage via regulating the balance of the excitatory/inhibitory amino acids to diminishing the excitatory toxic effect, and also promoting the release of monoamine transmitters. |