路鹏，励建安，刘元标，金挺剑，王骏.心肌短暂缺血后血管内皮生长因子表达的时间规律[J].中华物理医学与康复杂志,2004,(10):
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| 心肌短暂缺血后血管内皮生长因子表达的时间规律 |
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| DOI： |
| 中文关键词: 心肌缺血 血管内皮生长因子 时间规律 |
| 英文关键词: Myocardium ischemia VEGF Temporal patterns |
| 基金项目:国家自然科学基金资助项目（No.30370687） |
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| 中文摘要: |
| 目的观察心肌短暂缺血后血管内皮生长因子（VEGF）表达的时间规律。 方法阻断50只新西兰兔冠状动脉制作心肌缺血模型，分为对照组（S）、缺血组（0 h）及再灌注组（亚组：1 h，2 h，3 h，4 h，5 h，6 h，7 h）。采用Western Blotting法检测缺血和非缺血区心肌VEGF，酶联吸附试验检测外周血心肌肌钙蛋白（CTn-I）。 结果（1）再灌注5 h CTn-I无显著增高（P=0.863）。（2）缺血区心肌VEGF表达在缺血组与对照组差异无显著性意义；再灌注1 h显著升高，3 h达到高峰，4~7 h逐渐降低；非缺血区表现类似趋势，但时间滞后2~3 h。（3）再灌注各组缺血区VEGF表达均显著高于非缺血区，相关性良好。 结论VEGF表达在心肌缺血后3 h达到高峰，然后逐渐降低。非缺血区的改变与缺血区相关，但增加程度较低，时间相对滞后。 |
| 英文摘要: |
| Objective To study the temporal patterns of VEGF protein expression in rabbit myocardium subject to brief ischemia. MethodsFifty New Zealand rabbits were subjected to brief occlusion of left ventricle coronary artery (LVA) for 2 minutes. They were divided into a control group, an ischemic group and a reperfusion group which was further divided into sub-groups of 1h, 2h, 3h, 4h, 5h, 6h and 7h. Each group had five rabbits. Expression of VEGF protein in ischemic and non-ischemic myocardium was quantified by Western Blotting. Peripheral plasma samples were taken for CTn-I analysis. Results（1）CTn-I did not change significantly.（2）VEGF levels remained similar in ischemic myocardium in both control and ischemic groups. However, it increased significantly in the reperfusion groups at 1h, reached the peak at 3h, and decreased gradually from 4h to 7h. The time pattern in the non-ischemic myocardium was similar to that in the ischemic one, but delayed 2~3h afterwards. （3）VEGF level in ischemic myocardium was higher than the non-ischemic myocardium in the reperfusion groups with high correlation（r=0.983, P<0.001）. ConclusionThe expression of VEGF peaked at 3h in ischemic myocardium after transient ischemia. Temporal pattern in the non-ischemic myocardium was similar, but delayed. There was a close correlation with regard to VEGF expression between the ischemic and non-ischemic myocardium（r=0.983, P<0.001）. |
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