| Objective To explore the effect of hyperbaric oxygenation (HBO) on generation of free radicals and the permeability of blood-brain barrier(BBB) during cerebral ischemia-reperfusion(CIR). MethodsThree hundred and twenty Kunming mice were randomly divided into four groups: a sham operation group, a HBO group, a CIR group, and a HBO + CIR group, with 80 mice in each group. Conscious mice were used to establish the CIR model, and 0.25MPa (ATA) HBO were applied 5 times after operations. The activities of SOD, CAT, GSH-PX and the concentration of MDA, EB in the cerebral hippocampal tissues in each group were measured with colorimetry. The cerebral hippocampal tissues were harvested and processed, then observed and compared with transmission electron microscopic observation. ResultsCompared with the sham operation group, the activities of antioxidation enzymes (GSH-PX，SOD, CAT) in CIR group decreased significantly (P<0.01), while the concentration of MDA and EB increased significantly(P<0.01). Compared with the CIR group, the activities of antioxidation enzymes (GSH-PX，SOD, CAT)in the HBO + CIR group raised obviously, (P<0.01), while the concentration of MDA and EB lowered significantly(P<0.01). Compared with the sham operation group, the activity of GSH-PX in HBO group increased significantly (P<0.05). Meanwhile, in the CIR group, necrosis of neurons and edema of astrocyte were obvious, and the microtubule and microfilament of astrocyte decreased significantly. However, necrosis of neurons was not obvious in HBO + CIR group. ConclusionHBO could increase the activity of antioxidation enzymes, restore the function of astrocyte and decrease the permeability of BBB. |
