许涛，郭风劲，李新志，郭铁成，尤春景，陈安民.磁刺激对脊髓损伤组织c-fos基因表达的影响[J].中华物理医学与康复杂志,2003,(1):。-
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| 磁刺激对脊髓损伤组织c-fos基因表达的影响 |
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| DOI： |
| 中文关键词: 脊髓损伤 cfos基因 磁刺激 |
| 英文关键词: Spinal cord injury c-fos gene Magnetic stimulation |
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| 摘要点击次数: 1989 |
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| 中文摘要: |
| 目的观察磁刺激对损伤脊髓组织中cfos基因表达的影响。 方法用Allen氏WD法制造大鼠T8脊髓损伤的动物模型。38只Wistar大鼠随机分为正常组（6只）、脊髓损伤治疗组（16只）和对照组（16只）。治疗组于术后15 min、1 h、2 h和4 h分别予以磁刺激（0.5 Hz，70%最大输出强度），对照组无特殊处理。治疗组、对照组分别于术后15 min、1 h、2 h和4 h各取4只动物处死，取损伤段脊髓组织，作石蜡切片，用地高辛标记的cfos基因探针进行原位杂交，测定cfos mRNA在损伤脊髓组织中的表达，用免疫组织化学方法检测fos蛋白的表达。 结果fos蛋白OD值在正常组为3.78±0.72，在对照组明显增多（最高达16.49±1.3），而治疗组在各时段均低于对照组（P<0.01），cfos基因表达在正常脊髓组织中呈弱阳性，在对照组中表达明显增加，治疗组cfos基因表达的数目少于对照组（P<0.01）。 结论应用磁刺激能抑制脊髓损伤后cfos基因的表达，这可能是磁刺激保护神经元并减轻脊髓继发性损伤的机理之一。 |
| 英文摘要: |
| ObjectiveTo study the effects of magnetic stimulation (MS) on c-fos gene expression in spinal neurons of rats after spinal cord injury (SCI). MethodsThirty-eight Wistar rats were used, among them 32 rats underwent SCI with 10g×2.5cm impact at T8 level and were evenly divided into two groups: the MS group (n=16) and the control group (n=16), the other 6 rats served as the normal control. The rats in the MS group received 15 minutes, 1, 2 or 4 hours of MS at 0.5Hz and 70% of the maximal intensity output of the magnetic stimulator after the injury, while the rats in the other groups were not exposed to the MS. The c-fos mRNA levels of the rats were determined by in situ hybridization, the protein levels immunohistochemical methods. Results As compared with that in the normal controls (3.78±0.72), the c-fos protein expression increased significantly in rats with SCI in the control group (P<0.01), and this increase peaked at 2 hours after injury (16.49±1.30). The levels of c-fos expression in the MS group were significantly lower than those in the control group (P<0.01). Conclusion MS can inhibit the c-fos gene expression in spinal cord after injury, it may be one of the mechanisms that MS protects spinal cord against injury. |
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