王钢，刘世清.高压氧对鼠损伤脊髓内神经生长相关蛋白表达的影响[J].中华物理医学与康复杂志,2004,(12):
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| 高压氧对鼠损伤脊髓内神经生长相关蛋白表达的影响 |
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| DOI： |
| 中文关键词: 高压氧 脊髓损伤 神经生长相关蛋白 |
| 英文关键词: Hyperbaric oxygen Spinal cord injury GAP-43 |
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| 摘要点击次数: 1458 |
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| 中文摘要: |
| 目的研究高压氧（HBO）治疗对脊髓损伤（SCI）组织中神经生长相关蛋白（GAP-43）表达水平的影响。 方法用Allen氏WD法制作鼠脊髓损伤模型。55只成年Wistar鼠随机分为正常组、SCI对照组和HBO治疗组。治疗组于术后每天HBO治疗1次，对照组无特殊治疗。各组分别于术后1 d、4 d、7 d、10 d、14 d各随机取鼠5只，取损伤脊髓组织, Western blot法检测GAP-43表达水平。 结果GAP-43在正常成年鼠脊髓中少量表达，SCI后表达增加且HBO治疗组较对照组表达更为显著。术后1周时SCI对照组GAP-43表达达到高峰， 2周时降至接近初始水平，但HBO治疗组GAP-43表达仍维持高水平。 结论HBO可增强损伤脊髓组织GAP-43表达。 |
| 英文摘要: |
| Objective To investigate the effect of hyperbaric oxygen (HBO) on expression of GAP-43 in the rats spinal cord following spinal cord injury (SCI). MethodsFifty-five Wistar rats were randomly divided into three groups: a normal group, a control group and a HBO-treatment group. The rats in the control and HBO-treatment group underwent SCI by 10g×5cm impact at the T10 level. The rats in the HBO-treatment group were treated by HBO. Western blot was used to observe the change of expression of GAP-43. ResultsThere was weak expression of GAP-43 in the normal spinal cord. The GAP-43 expression increased significantly following SCI. The level of GAP-43 expression in the HBO-treatment group was significantly high than that in the control group. ConclusionHBO can increase the level of GAP-43 expression. |
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