王鑫，孙彩花，钱贞，施伟，孙志勇，杨敏，胡喆.丰富环境对卒中后认知障碍小鼠认知功能及突触可塑性的影响[J].中华物理医学与康复杂志,2016,38(9):647-651
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| 丰富环境对卒中后认知障碍小鼠认知功能及突触可塑性的影响 |
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| DOI： |
| 中文关键词: 卒中后认知障碍 丰富环境 突触可塑性 |
| 英文关键词: Stroke Cognitive impairment Enriched environments Synaptic plasticity |
| 基金项目:国家自然科学基金资助项目（81301673） |
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| 中文摘要: |
| 目的探讨丰富环境干预对卒中后认知障碍小鼠认知功能的影响及相关作用机制。 方法采用光栓法制作小鼠卒中后认知障碍模型，采用随机数字表法将实验小鼠分为假手术标准环境组（Sham+SE组）、卒中后认知障碍标准环境组（PSCI+SE组）和卒中后认知障碍丰富环境组（PSCI+EE组）。各组小鼠在相应环境下分别饲养28d后，采用水迷宫检测小鼠认知功能，采用电生理方法检测小鼠海马长时程增强，采用Western blot法检测海马突触素表达情况，采用电镜定量检测海马CA1区神经元突触超微结构变化。 结果与Sham+SE组小鼠比较，PSCI+SE组小鼠水迷宫成绩显著下降（P<0.05），卒中对侧海马长时程增强诱导障碍（P<0.05），海马CA1区突触素表达显著降低（P<0.05），海马CA1区神经元突触数量、突触间隙宽度、突触后膜致密物厚度及突触界面曲率均发生明显不良改变（P<0.05）；PSCI+EE组小鼠水迷宫成绩、海马长时程增强、CA1区突触素表达、CA1区神经元突触结构均较PSCI+SE组有显著改善（P<0.05），但与Sham+SE组间差异仍具有统计学意义（P<0.05）。 结论丰富环境干预可改善卒中后小鼠认知功能，其作用机制与提高卒中对侧海马突触可塑性有关。 |
| 英文摘要: |
| Objective To observe the effects of an enriched environment (EE) on cognitive functioning and the synaptic plasticity of mice modeling post-stroke cognitive impairment (PSCI) and explore the possible mechanisms involved. MethodsMice modeling PSCI and sham-operated mice were randomly divided into 3 groups: sham-operated mice in a standard environment (the Sham+SE group), PSCI mice in a standard environment (the PSCI+SE group) and PSCI mice in an enriched environment (the PSCI+EE group). The cognitive functioning of all of the mice was quantified using a Morris water maze and their hippocampal long-term potentiation (LTP) was recorded using an electrophysiological method. The level of synaptophysin was detected using Western blotting. Synaptic ultrastructure in the hippocampus was imaged using electron microscopy. ResultsCompared with the Sham+SE group, the PSCI+SE group showed significantly poorer water maze performance and failed induction of contralateral LTP. Their average level of synaptophysin was significantly lower, and significant adverse changes in the synaptic ultrastructure of the hippocampus were observed, including a decreased number of synapses. The average width of the synaptic cleft, postsynaptic density and the interface curvature of the synapses were all less desirable. All of the measurements of the PSCI+EE group improved significantly compared to those of the PSCI+SE group, but were still significantly poorer than those of the Sham+SE group. ConclusionsAn enhanced environment can improve the cognitive functioning of mice modelling PSCI. It may be that an EE can improve synaptic plasticity in the hippocampus contralateral to the stroke. |
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