王勇，任佰绪，吴书峰，钟琴，李小俚，路承彪.经颅超声刺激对帕金森小鼠运动功能及抗氧化能力的影响[J].中华物理医学与康复杂志,2015,37(7):488-492
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| 经颅超声刺激对帕金森小鼠运动功能及抗氧化能力的影响 |
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| DOI： |
| 中文关键词: 经颅超声刺激 帕金森病 丙二醛 谷胱甘肽过氧化物 |
| 英文关键词: Ultrasound Parkinson′s disease Motor function Antioxidants Brain |
| 基金项目:国家自然科学基金(31070938; 81271422)；河北省应用基础研究计划重点基础研究(12966119D) |
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| 中文摘要: |
| 目的探讨经颅超声刺激(TUS)对帕金森病(PD)小鼠运动功能及抗氧化能力的影响。 方法选择近交系C57BL雄性小鼠32只，按照随机数字表法将其分为正常对照组、模型组、假超声刺激组、超声刺激组，每组8只。模型组、假超声刺激组、超声刺激组采用1-甲基-4苯基-1,2,3,6-四氢吡啶(MPTP)腹腔注射(20mg/kg)制备PD模型，正常对照组给予生理盐水。造模成功后，采用0.5MHz、1W/cm2低强度聚焦超声波(LIFU)穿过超声刺激组小鼠颅骨刺激中脑黑质区，将超声探头置于假超声刺激组小鼠头皮上、关闭超声波。造模前、造模2周后、造模5周后对各组小鼠进行爬杆测试。造模5周后处死大鼠，测定全脑丙二醛(MDA)、谷胱甘肽过氧化物(GSH-Px)含量。 结果造模前，各组小鼠运动功能评分之间比较，差异无统计学意义(P>0.05)。与组内造模前比较，模型组［(4.30±1.19)分］、假超声刺激组［(4.40±0.23)分］、超声刺激组［(4.80±0.23)分］造模2周后运动功能评分显著降低(P<0.05)，模型组［(5.12±0.83)分］、假超声刺激组［(5.51±1.21)分］造模5周后运动功能评分亦较低(P<0.05)。与组内造模2周后比较，超声刺激组造模5周后运动功能评分［(6.69±1.11)分］显著升高(P<0.05)。与正常对照组比较，其余3组造模2周后运动功能评分较低(P<0.05)，模型组、假超声刺激组造模5周后运动功能评分较低(P<0.05)。与模型组比较，超声刺激组造模5周后运动功能评分较高(P<0.05)。与假超声刺激组比较，超声刺激组造模5周后运动功能评分较高(P<0.05)。造模5周后，模型组［(10.2±1.1)nmol/ml］、假超声刺激组［(9.4±1.3)nmol/ml］MDA含量较正常对照组［(4.5±0.8)nmol/ml］显著升高(P<0.05)，超声刺激组MDA含量［(6.8±0.9)nmol/ml］较模型组、假超声刺激组降低(P<0.05)。造模5周后，模型组［(100.0±35.4)U/mgprot］、假超声刺激组［(444.0±24.9)U/mgprot］GSH-Px酶活力水平较正常对照组［(1262.5±53.0)U/mgprot］显著降低(P<0.05)，超声刺激组［(1047.3±77.8)U/mgprot］GSH-Px酶活力水平较模型组、假超声刺激组升高(P<0.05)。 结论TUS可改善PD小鼠的运动功能，其机制可能与TUS增强其抗氧化能力有关。 |
| 英文摘要: |
| Objective To investigate the effects of transcranial ultrasound stimulation (TUS) on the motor functioning and anti-oxidative capacity of mice with Parkinson′s disease (PD). MethodsThirty-two inbred C57BL male mice were randomized into a normal control group, a model group, a sham TUS group and a TUS group (n=8 for each group) according to a random number table. A PD model was induced in the mice of the model, sham TUS and TUS groups by injecting 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 20 mg/kg intraperitoneally, while those in the normal control group were given saline. Low intensity (1 W) focused ultrasound (LIFU) at a frequency of 0.5 MHz was then applied to stimulate the nigra region, except for the mice in the sham TUS group, which were treated with the same procedure but with no ultrasound output. A pole climbing test was carried out before, 2 weeks and 5 weeks after the injection of the MPTP. After 5 weeks the animals were sacrificed and the whole brain malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) content were measured. ResultsNo significant differences in in pole climbing scores were observed among the four groups before the MPTP injections. However, the average value decreased significantly to (4.30±1.19), (4.40±0.23) and (4.80±0.23) for the model, sham TUS and TUS groups respectively 2 weeks after the injection. It then rose to (5.12±0.83) and (5.51±1.21) for the first two groups 3 weeks later, but was still lower that before the injection. After 5 weeks the TUS group′s average score was significantly higher than 3 weeks earlier and than that of the model group and the sham TUS group. Compared with the control group, the other groups′ average scores were all lower 2 weeks after MPTP injection, and those of the model and the sham TUS groups remained so 5 weeks after the injection. Five weeks after the injection, the average MDA content of the model group (10.2±1.1 nmol/ml) and the sham TUS group (9.4±1.3 nmol/ml) were significantly higher than the normal control group (4.5±0.8 nmol/ml), as well as the TUS group (6.8±0.9 nmol/ml). However, GSH-Px enzyme activity in the model group (100±35.4 U/mgprot) and the sham US group (444±24.9 U/mgprot) was significantly lower than that in the normal control group (1262.5±53 U/mgprot), together with the TUS group (1047.3±77.8 U/mgprot). ConclusionTUS can improve motor function in PD, at least in mice. This may be due to its anti-oxidative capacity. |
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