洪新如，卢晓欣，陈新民.脑源性神经营养因子对新生大鼠缺氧缺血性脑损伤后内源性阿片肽的影响[J].中华物理医学与康复杂志,2004,(6):
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| 脑源性神经营养因子对新生大鼠缺氧缺血性脑损伤后内源性阿片肽的影响 |
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| DOI： |
| 中文关键词: 神经营养因子 阿片肽类 脑缺氧 脑缺血 新生大鼠 |
| 英文关键词: Neurotrophic factor Opioid peptides Brain hypoxia-ischemia Rat, newborn |
| 基金项目:全军“九?五”医学科研规划科研基金资助项目（No.98D022） |
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| 中文摘要: |
| 目的观察新生大鼠缺氧缺血性脑损伤（HIBI）后，外源性脑源性神经营养因子（BDNF）对脑内源性阿片肽（EOP）水平的影响。 方法7 d龄新生大鼠随机分为HIBI+BDNF组（A组）、单纯HIBI组（B组）和假手术组（C组）。结扎幼鼠左侧颈总动脉并在低氧（8%O2+92%N2）环境下形成HIBI，造模后立刻向侧脑室注射0.5 μg BDNF，给药后即刻、60 min及120 min观察左、右侧皮层和海马强啡肽A1-13免疫活性物质（ir-DynA1-13）、β-内啡肽免疫活性物质（ir-β-EP）和亮啡肽免疫活性物质（ir-LEK）含量的变化。 结果①各组新生大鼠HIBI后大多数时间点相比，B组皮层、海马ir-DynA1-13和ir-β-EP含量较C组显著升高（P<0.05或P<0.01）；伤后即刻，B组左侧皮层、海马ir-LEK含量显著高于C组（P<0.05）。②给予BDNF后，A、B组ir-DynA1-13含量在大多数时间点与C组比较，差异有显著性意义（P<0.05或P<0.01）；皮层在给药后60 min，A组ir-β-EP含量显著低于B组（P<0.05），其余各时间点与B组比较，差异均无显著性意义；A、C组比较，海马ir-LEK含量差异均无显著性意义。 结论脑内EOP中，以ir-DynA1-13、ir-β-EP与HIBI关系更为密切。通过影响强啡肽与内啡肽系统对抗HIBI是BDNF的作用途径之一。 |
| 英文摘要: |
| Objective To explore the effects of brain-derived neurotrophic factors (BDNF) on concentrations of cerebral endogenous opioid peptides(EOP)in neonatal rats subject to with hypoxic ischemic brain injury (HIBI). MethodsSeven-day-old Sprague Dawley rats were randomly divided into a HIBI+BDNF group (group A),a HIBI group (group B) and a sham-operation group (group C). Models of HIBI were established by use of permanent ligation of the left common carotid artery followed by 2.5 hours ofinhalation of 8%O2+92%N2, then 0.5 μg BDNF was injected into the parietal cerebral ventricle in group A immediately. Contents of dynorphin A1-13-like, β-endorphin-like and leu-enkaphalin-like immunoreactivities (ir-DynA1-13, ir-β-EP and ir-LEK) in cortex and hippocampus were measured at 0, 60, 120 min after administration of BDNF. ResultsThe concentrations of ir-DynA1-13 and ir-β-EP in the cortex and hippocampus in group B were increased significantly than those in group C at most time points（P<0.05 or P<0.01）. The contents of ir-LEK in left cortex and hippocampus in group B were significantly higher at 0 min after injury（P<0.05）. After administration of BDNF, there were significant difference between the contents of ir-DynA1-13 in groups A and C at most time points except for those in hippocampus at 60 min and/or 120min(P<0.05 or P<0.01).The content of ir-β-Ep in the cortex in group A was significantly lower than that in group B at 60 min(P<0.05), while there was no significant difference between both groups at other time points. There was no significant difference between groups A and C with regard to the contents of ir-LEK in hippocampus. Conclusion Among 3 kinds of EOPs, ir-DynA1-13 and ir-βEp might be more closely related to HIBI. HIBI could be alleviated by exogenous BDNF with effects on Dynorphin and Endorphin systems. |
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