李靖，陈一冰，张颖.运动对去势雌性大鼠骨质疏松的保护作用[J].中华物理医学与康复杂志,2003,(12):.-
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| 运动对去势雌性大鼠骨质疏松的保护作用 |
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| DOI： |
| 中文关键词: 运动 骨质疏松 凋亡 胰岛素样生长因子-1 骨细胞 |
| 英文关键词: Exercise Osteoporosis Apoptosis IGF-1 Osteocyte |
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| 中文摘要: |
| 目的探讨运动训练对去势雌性大鼠骨质疏松的保护作用及相关机理。 方法将24只6月龄雌性大鼠分为3组：对照组、去势组及运动组。去势组和运动组大鼠均行双侧卵巢切除手术，对照组为假手术组。对照组和去势组大鼠术后正常活动，运动组大鼠术后则在跑台上进行中等强度运动训练（跑步速度为18 m/min，每天1 h，每周训练5 d）。各组大鼠分别于术后12周时处死并对其股骨骨密度（bone mineral density, BMD）进行测定；对股骨转子区域骨组织进行胰岛素样生长因子（insulin-like growth factor-1，IGF-1）免疫组织化学染色；并结合计算机图像分析系统对骨细胞IGF-1表达强度进行评定；应用原位凋亡法检测股骨转子区骨细胞凋亡。 结果对照组、去势组及运动组BMD分别为（0.170±0.011）g/cm2，（0.154±0.013）g/cm2和（0.167±0.013）g/cm2，运动组BMD与对照组比较差异无显著性，去势组BMD显著低于对照组及运动组(P均<0.05)。运动组骨细胞IGF-1表达强度显著高于对照组及去势组(P均<0.05)，去势组骨细胞IGF-1表达强度与对照组比较差异无显著性。对照组、去势组及运动组的骨细胞凋亡率分别为2.1%，5.3%及1.8%，运动组骨细胞凋亡率与对照组比较差异无显著性，去势组骨细胞凋亡率显著高于对照组及运动组(P均<0.01)。 结论大鼠雌激素缺乏可使其骨细胞凋亡数目增加。运动可缓解因雌激素缺乏引起的骨质疏松，其机理可能与运动抑制骨细胞凋亡、促进骨细胞IGF-1表达有关。 |
| 英文摘要: |
| Objective To investigate the protective effect of exercise on osteopenia female rats caused by ovariectomy and its mechanism. MethodsTwenty-four female rats aged 6 months were randomly divided into 3 groups: control group(CON),ovariectomized model group(OVX) and exercise group(Ex).The ovariectomy operation was performed with rats in OVX and Ex groups. Rats in Ex group were trained on a treadmill at a velocity of 18m/min for 1h, once daily for 5 days a week. Each group of rats were sacrificed at 12 weeks postoperatively and Dual-energy X-ray absorptiometry was carried out to measure the bone mineral density(BMD) of the femur. IGF-1 proteins in trabecular bone of proximal femur were examined by using immunochemical stain and the intensity of IGF-1 was determined by use of imaging analysis techniques. The apoptotic osteocytes in the trabecular bone of proximal femur were detected by using terminal-deoxynucleotidyl mediated nick end labeling (TUNEL) technique. ResultsThe BMD of CON, Ex and OVX group was （0.170±0.011）g/cm2，（0.154±0.013）g/cm2 and（0.167±0.013）g/cm2, respectively. The difference of the BMD level between Ex and CON groups was not significant, however, the BMD level in OVX group was significantly lower than that of the Ex and CON groups (P<0.05). Immunochemistry stain of IGF-1 was more intensive in osteocytes of Ex group than that of CON and OVX groups(P<0.05),however, no difference was found between CON and OVX groups. The apoptotic rate of osteocytes in CON, Ex and OVX groups was 2.1%，5.3% and 1.8%, respectively. Apoptotic osteocyte in trabecular bone was significantly higher in the OVX group when compared with the CON and Ex groups(P<0.01).No statistical difference in apoptotic osteocytes was found between the CON and Ex group. ConclusionIncrement of apoptotic osteocytes was detected in rats with estrogens deficiency. Exercise could inhibit the apoptosis of osteocytes and increase the expression of IGF-1 in osteocytes, it may be one of the mechanisms that exercise protects the rats with estrogens deficiency from osteoporosis. |
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