宫阳阳、侯梅、苑爱云、李小平、张大伟.早期运动干预对脑缺血缺氧幼鼠海马区突触素蛋白表达的影响[J].中华物理医学与康复杂志,2016,38(5):325-328
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| 早期运动干预对脑缺血缺氧幼鼠海马区突触素蛋白表达的影响 |
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| DOI： |
| 中文关键词: 海马 脑损伤 突触素 尼氏体 鼠 |
| 英文关键词: Hippocampus Brain Injury Synaptophysin Nissl bodies Rats |
| 基金项目:山东省医药卫生科技发展计划项目（2015WS0352）；青岛市卫生科技计划项目（2015-WJZD091）；青岛市医疗卫生优秀人才培养项目 |
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| 中文摘要: |
| 目的 观察早期运动干预对缺血缺氧性脑损伤（HIBD）幼鼠大脑海马区神经元突触素(Syn)蛋白表达及尼氏体的变化，探讨HIBD早期运动干预的疗效机制。 方法 取3窝新生7d的幼鼠35只，按随机数字表法分为对照组（11只）、干预组（13只）和假手术组（11只）。制作新生Wistar大鼠幼鼠HIBD模型。干预组造模成功后7d开始早期运动干预，每天游泳10min，持续14d；对照组只造模不进行运动干预；假手术组只分离左侧颈总动脉但不结扎，不进行运动干预。采用免疫印迹（Western blot）法检测大脑海马区Syn蛋白的表达水平，以Syn和β带灰度值的比值表示syn蛋白相对表达量；采用尼氏染色方法依据形态学结果观察幼鼠海马区神经元尼氏体数量和细胞形态。 结果 对照组病灶侧海马组织Syn表达(0.447±0.141)较假手术组左侧海马组织Syn表达(1.027±0.035)显著降低(P<0.01)、海马区神经元尼氏体明显减少；干预组病灶侧海马区Syn表达(0.772±0.121)较对照组病灶侧海马组织Syn表达(0.447±0.141)明显升高(P<0.05)、海马区神经元尼氏体显著增多。 结论 早期运动干预可增强缺血缺氧幼鼠病灶侧海马区Syn蛋白的表达和增加神经元内尼氏小体数量。 |
| 英文摘要: |
| Objective To observe the effects of early exercise on the expression of synaptophysin protein and Nissl bodies in the hippocampus after hypoxic-ischemic brain damage(HIBD), and to investigate possible mechanisms. Methods A total of 35 neonatal Wistar rats aged 7 days were randomly divided into a training group of 13, a control group of 11 and a sham-operation group of 11. HIBD was induced in the rats of the training and control groups, while those in the sham-operation group had the left common carotid artery separated, but without ligation. Seven days after successful modeling, the training group began swimming training for 10 min every day lasting for 14 days, while the other groups were not trained. Western blotting was used to detect the expression of synaptophysin in the affected hippocampus of the brain based on the ratio of the gray band values for synaptophysin and beta. Nissl staining was applied to observe the number of Nissl bodies and the morphology of the neurons in the hippocampus. Results The average expression of synaptophysin in the sinistrocerebral hippocampus of the rats in the control group was significantly lower than that in the sham-operation group, but significantly higher than that of the training group. The control group had significantly fewer Nissl bodies than the sham-operation group, but significantly more than the training group. Conclusion Early training can increase the expression of synaptophysin and the number of Nissl body in the hippocampus after hypoxic-ischemic brain damage. |
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