孙国剑，马军廷，李占标，王强.游泳训练对脑梗死大鼠大脑皮质中神经生长因子及神经营养因子-3表达的影响[J].中华物理医学与康复杂志,2015,37(8):561-564
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| 游泳训练对脑梗死大鼠大脑皮质中神经生长因子及神经营养因子-3表达的影响 |
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| DOI： |
| 中文关键词: 运动训练 脑缺血再灌注 神经再生 神经生长因子 神经营养因子-3 |
| 英文关键词: Exercise training Cerebral ischemia-reperfusion Neural regeneration Nerve growth factor Neurotrophins-3 |
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| 中文摘要: |
| 目的观察游泳训练对脑梗死大鼠神经生长因子（NGF）及神经营养因子-3（NT-3）表达的影响，并初步探讨运动训练对脑梗死大鼠的神经保护机制。 方法共选取健康雄性SD大鼠45只，采用随机数字表法将其分为假手术组、对照组及训练组。采用线栓法将对照组及训练组大鼠制成左侧大脑中动脉阻塞（MCAO）2h再灌注模型，假手术组大鼠制模期间不阻塞大脑中动脉血流。训练组大鼠制模后给予游泳训练，每日1次，每次持续10min，对照组及假手术组大鼠制模后不给予任何特殊处理。于制模后3d、7d及14d时采用Bederson评分法评定各组大鼠神经功能缺损情况；采用RT-PCR法检测各组大鼠缺血侧脑皮质NGF及NT-3 mRNA表达量。 结果假手术组大鼠术后无神经行为缺陷，制模后3d、7d及14d时训练组大鼠Bederson评分均显著低于同时相点对照组水平（P<0.05），高于同时相点假手术组水平（P<0.05），并且制模后14d时训练组Bederson评分［（1.20±0.45）分］亦显著低于制模后3d及7d时水平（P<0.05）。训练组各时相点缺血侧脑皮质NGF及NT-3 mRNA表达量均较对照组、假手术明显增强（P<0.05）；随着时间进展，制模后14d时训练组NGF及NT-3 mRNA含量［分别为（0.66±0.07），（0.79±0.06）］均较制模后3d及7d时明显提高（P<0.05）。 结论游泳训练能上调脑梗死大鼠缺血脑皮质NGF及NT-3 mRNA表达，这可能是运动训练促进脑梗死大鼠受损神经功能恢复的重要机制之一。 |
| 英文摘要: |
| Objective To observe the influence of swimming training on the expression of nerve growth factor (NGF) and neurotrophins-3 (NT-3) in rats with cerebral infarction, and to explore the underlying neuroprotection mechanism of exercise training on cerebral infarction. MethodsForty-five healthy male Sprague-Dawley rats were randomly divided into a sham-operation group, a control group and a training group, with 15 rats in each group. Each group was further divided into a 3-day, 7-day and 14-day subgroups, which amounts to 9 groups. To establish animal model of cerebral ischemia-reperfusion in rats, the intraluminal thread method was applied to cause left middle cerebral artery occlusion (MCAO) for 2 h before reperfusion. The rats of the training group were given swimming training for 10 min, once daily, while those of the sham-operation and control groups were not given any training. Neurological deficits were assessed using Bederson scores. The expression of NGF mRNA and NT-3 mRNA in the ischemia-reperfusion pallium was examined using reverse transcription-polymerase chain reaction (RT-PCR). ResultsThe rats of the sham-operation group showed no neurological deficits. At the same time points, the average Bederson scores of the training group were significantly lower than the control group, but significantly higher than the sham group. Moreover, the 14 d training group had the lowest Bederson score (1.20±0.45), compared to the value 3 and 7 days after modeling. The expression of NGF mRNA and NT-3 mRNA of ischemic cerebral cortex in the training group was significantly improved when compared to the sham-operation group or the control group. On day 14, the expression of the NGF mRNA (0.66±0.07), and the NT-3 mRNA (0.79±0.06), were significantly higher than those on day 3 and 7. ConclusionsSwimming training could increase the expressions of NGF mRNA and NT-3 mRNA in the ischemic cerebral cortex. It might be one of the key mechanisms that exercise training could promote the recovery of damaged neurological function in rats with cerebral infarction. |
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