刘志华，左玲，苗贵申，王珺楠，杨聪娴，傅志俭，孙涛.消退素D1对突出髓核所致根性神经痛的影响[J].中华物理医学与康复杂志,2016,38(11):819-824
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| 消退素D1对突出髓核所致根性神经痛的影响 |
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| DOI： |
| 中文关键词: 消退素 腰椎间盘突出症 根性神经痛 ERK NF-κB/p65 |
| 英文关键词: Resolvin D1 Lumbar disc herniation Radicular pain ERK NF-κB/p65 |
| 基金项目:国家自然科学基金（81271232）；山东省科技发展计划（2014GSF118130）；国家临床重点专科建设项目经费资助 |
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| 中文摘要: |
| 目的研究消退素D1对突出髓核所致根性神经痛的影响及内在机制。 方法选取56只雄性Sprague-Dawley（SD）大鼠，按照随机数字表法分为假手术组、模型组、消退素D1 10ng组、消退素D1 100ng组，每组14只，建立非压迫性椎间盘突出症模型。术后1d、2d、3d，假手术组和模型组分别注射磷酸盐缓冲液（PBS）10μl，消退素D1 10ng组和消退素D1 100ng组分别注射含有消退素D1 10ng和100ng的PBS 10μl。于术前1d及术后连续7d分别检测各组大鼠术侧后肢50%缩足阈值（PWT）。术后7d，取大鼠脊髓组织经酶联免疫吸附测定法（ELISA）检测肿瘤坏死因子（TNF-α）、白介素1β（IL-1β）和白介素10（IL-10）的蛋白表达量，采用蛋白质印记Western blot法检测脊髓组织中细胞外信号调节激酶（ERK）核转录因子NF-κB/p65（NF-κB/p65）的含量变化。 结果术后1d至7d，模型组大鼠50% PWT较假手术组明显降低（P<0.05）；术后3d、4d、5d、6d、7d，消退素10ng组与模型组比较，50% PWT显著升高（P<0.05）；术后2d、3d、4d、5d、6d、7d，消退素100ng组与模型组比较，50% PWT显著升高（P<0.05）。与假手术组比较，模型组大鼠TNF-α和IL-1β的蛋白表达水平明显升高（P<0.05），IL-10的表达水平明显降低（P<0.05）；与模型组比较，消退素10ng组和消退素100ng组的TNF-α和IL-1β的蛋白表达水平明显降低（P<0.05），IL-10的表达水平明显升高（P<0.05），且消退素100ng组的蛋白含量下降更显著（P<0.05）。与假手术组比较，模型组p-ERK、NF-κB/p65蛋白水平明显升高（P<0.05）；与模型组比较，消退素10ng组和消退素100ng组p-ERK及NF-κB/p65蛋白水平明显降低（P<0.05），且消退素100ng组降低的更明显（P<0.05）。 结论消退素D1通过调节炎症介质表达及p-ERK和NF-κB/p65通路的活性，促进神经根炎症的消退，为治疗腰椎间盘突出症提供了新的思路。 |
| 英文摘要: |
| Objective To investigate the analgesic effect of Resolvin D1 (RvD1) on radicular pain induced by herniated nucleus pulposusand its underlying mechanism. MethodsFifty-six male rats were randomly divided intoa sham group, a model group, a 10 ng group anda 100 ng group, each of 14. The rat model of non-compressive lumber disc herniation was established in all except the sham group. The former two groups were then injected with 10 μl of phosphate buffer solution (PBS) while the latter 2 groups were injected with 10 μl of PBS containing 10 and 100 ng of RvD1 respectively daily for three successive days. The rats′ 50% paw withdrawal threshold (PWT) was evaluated 1 day before and on 7 successive days after surgery. On day 7 the rats′ spinal cords were removed to assess the expression of tumor necrosis factor-α(TNF-α), interlukin-1β(IL-1β) and interlukin-10 (IL-10) using ELISA methods. The levels of ERK and NF-κB/p65 were measured using Western blotting. ResultsTheaverage 50%PWT of the model group decreased significantly from day 1 to day 7 compared with the sham group, but was significantly lower thanthe RvD1 10 ng group from day 3 to day 7. Moreover the 50%PWT in the RvD1 100 ng group increased significantlyfrom day 2 to day 7 compared with the model group (P<0.05). The average expression of both TNF-α and IL-1β of the model group was upregulated significantly and that of IL-10 decreased significantly compared with the sham group. Compared with the model group, the expression of TNF-α and IL-1β decreased significantly (P<0.05) and the level of IL-10 was significantlyup-regulated (P<0.05) both in the RvD1 10 ng group and 100 ng group. Moreover, the changes were larger in the RvD1 100 ng group (P<0.05). Compared with the sham group, the levels of p-ERK and NF-κB/p65 in the model group were significantly up-regulated (P<0.05). Compared with the model group, intrathecal injection of RvD1 (10 ng or 100 ng) significantly decreased the expressions of p-ERK and NF-κB/p65 (P<0.05). Moreover, the decrease wasgreater in the RvD1 100 ng group (P<0.05). ConclusionsRvD1 might alleviate the radicular inflammation and pain byregulating the balance of inflammatory mediators and activation of p-ERK and NF-κB/p65 pathways. It may offer novel therapeutic approaches for the management of lumbar disc herniation. |
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