刘宇炜，余达经，刘仁刚，刘云华，周洁萍.高温对HeLa细胞张力原纤维和微管纤维的影响[J].中华物理医学与康复杂志,2003,(11):.-
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| 高温对HeLa细胞张力原纤维和微管纤维的影响 |
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| DOI： |
| 中文关键词: 高温 HeLa细胞 张力原纤维 微管纤维 |
| 英文关键词: Hyperthermia HeLa cells Tonofibril Microtubule |
| 基金项目:国家自然科学基金资助项目（N0.59836240） |
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| 摘要点击次数: 2048 |
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| 中文摘要: |
| 目的研究高温对肿瘤细胞张力原纤维和微管纤维的影响。 方法将HeLa细胞分成8组，置于不同温度（37，40，43和45℃）下，分别经不同时间（1 h和2 h）水浴处理后，倒置显微镜下观察其形态变化，然后用考马斯亮蓝R250显示其张力原纤维，SABC法显示其微管纤维。 结果当温度由40℃升至43℃的过程中，首先引起HeLa细胞张力原纤维的解聚，进而引起微管纤维的解聚，随着温度的升高和时间的延长，两者的变化逐渐趋同。而微管组织中心具有较强的保护作用。 结论温和性高温对HeLa细胞张力原纤维的影响可能强于微管纤维，两者都呈现出渐进式的解聚变化，这些变化都和高温导致的细胞毒性及凋亡有着非常密切的关系。 |
| 英文摘要: |
| Objective To investigate the effects of hyperthermia on tonofibrils and microtubules of tumor cells. Methods HeLa cells were divided into eight groups, placed respectively in 37℃, 40℃, 43℃ and 45℃, incubated for 1h or 2hs. Inverted microscope was used to observe its morphologic changes, Coomassie Blue R250 and SABC method were used to show its tonofibrils and microtubules. ResultsHyperthermia led to depolymerization of tonofibrils of HeLa cells earlier, then depolymerization of microtubules in the process of temperature ascending from 40℃ to 43℃.As the temperature decreases and the action time prolongs, both changes tend to become the same. The microtubule organizing center(MTOC) had powerful protection functions. ConclusionThe mild hyperthermia exerted stronger effects on tonofibrils than on microtubules; Under the hyperthermia, both tonofibrils and microtubules presented gradually changes of depolymerization, which closely related with cytotoxicity and apoptosis induced by heat. |
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