任慧聪，张朝辉，赵琳，谷世娜，李文强，宋景贵.重复经颅磁刺激对慢性应激抑郁模型大鼠行为学及HPA轴的影响[J].中华物理医学与康复杂志,2016,38(9):664-668
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| 重复经颅磁刺激对慢性应激抑郁模型大鼠行为学及HPA轴的影响 |
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| DOI： |
| 中文关键词: 抑郁症 重复经颅磁刺激 下丘脑-垂体-肾上腺轴 慢性应激 |
| 英文关键词: Depression Transcranial magnetic stimulation Hypothalamus-pituitary-adrenal axis Stress |
| 基金项目:国家自然科学基金（3110086）；河南省医学科技攻关计划项目（201003071）；新乡市科技攻关计划项目（ZG15019） |
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| 中文摘要: |
| 目的观察重复经颅磁刺激（rTMS）对慢性应激抑郁模型大鼠行为学的影响，并探讨其可能的神经内分泌机制。 方法筛选40只同质均一的健康成年雄性SD大鼠按随机数字表法分为对照组（8只）和造模组（32只）。对照组正常饲养条件下群养，每笼4只，不给予任何干预处理；造模组大鼠采用联合慢性温和不可预知应激（CUMS）方法孤养制备大鼠抑郁模型。运用体重测量、蔗糖水消耗实验及旷场实验行为学评估指标筛选出造模成功的大鼠，将造模成功的大鼠再按随机数字表法分为模型组、rTMS组和伪刺激组，每组8只大鼠。rTMS组和伪刺激组分别接受rTMS和假rTMS刺激干预，为期3周。分别于造模后和干预结束后，运用上述行为学评估指标对各组大鼠行为学改变进行评估，应用酶联免疫吸附实验（ELISA）检测大鼠血浆促肾上腺皮质激素（ACTH）和皮质酮（CORT）的含量，采用半定量逆转录聚合酶链式反应（RT-PCR）检测下丘脑促肾上腺皮质激素释放激素（CRH）mRNA表达量。 结果①CUMS处理后（造模后）大鼠体重增长率［（24.784±13.879）%］、蔗糖水消耗量［（2.102±0.998）ml/100g］、旷场实验垂直运动次数［（3.500±2.207）次］及修饰次数［（8.792±2.889）次］均较对照组［（39.810±11.739）%、（3.510±0.878）ml/100g、（10.875±4.357）次、（18.125±4.853）次］显著降低，差异有统计学意义(P<0.01)；rTMS干预后，与模型组相比，rTMS组大鼠体重增长率［（36.520±6.469）%］、蔗糖水消耗量［（4.130±0.917）ml/100g］、旷场实验的垂直运动次数［（7.000±3.742）次］及修饰次数［（16.250±3.012）次］均显著增高(P<0.05)；②干预后，模型组大鼠血浆ACTH和CORT含量［（204.014±13.915）和（203.053±18.502）μg/L］显著高于对照组［（108.705±12.808）和（104.611±5.099）μg/L］，而rTMS组的ACTH和CORT水平［（131.964±12.172）和（129.378±8.806）μg/L］均明显较模型组降低，且差异有统计学意义(P<0.01)；③与对照组相比，模型组大鼠下丘脑CRH mRNA的表达显著增高［（1.92±0.18）%］,差异有统计学意义(P<0.01)；而rTMS组下丘脑CRH mRNA表达较模型组有明显降低［（1.12±0.12）%］,差异有统计学意义(P<0.01)。 结论rTMS刺激能够改善慢性应激抑郁模型大鼠的抑郁样行为，其机制可能与下调亢进的下丘脑-垂体-肾上腺（HPA）轴有关。 |
| 英文摘要: |
| Objective To observe the effect of repetitive transcranial magnetic stimulation (rTMS) on behavior in response to chronic but unpredictable mild stress and explore potential neuroendocrine mechanisms. MethodsForty adult SD male rats were randomly divided into a control group (n=8) and a model preparation group (n=32). The control group was given normal care while a model of depression was induced in the model preparation group through giving an unpredictable mild stimulus (CUMS). The depressive rats were randomly divided into a model group, an rTMS group and a sham rTMS group (8 cases in each group). The rTMS group and sham rTMS groups accepted the rTMS or sham stimulation for 3 weeks. The changes in behavior in each group were quantified using body weight, sucrose consumption and an open field test before and after stimulation. Enzyme-linked immunosorbent assays (Elisas) were conducted to detect plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels. Reverse-transcription polymerase chain reactions (RT-PCRs) were carried out to allow the detection of mRNA expression in hypothalamus related to levels of adrenocorticotropic hormone releasing hormone (CRH). Results After the modeling there were significant differences between the model preparation group and the control group in terms of weight increase, sucrose consumption and open field test results. After rTMS the rate of weight increase, sucrose consumption and the scores in the open field test of the rTMS group had increased significantly more than in the control group. Elisas showed significantly higher plasma ACTH and CORT levels in the model group as well. The average expression of CRH mRNA in the model group was significantly higher than in either of the other two groups. ConclusionsrTMS can relieve depression-like behavior induced by chronic stress, at least in rats. This may be related to a downgrading of the hyperactive functioning of the hypothalamus-pituitary-adrenal axis. |
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