文章摘要
王伟欢,代玉玺,吴卫东,等.中等强度持续运动与高强度间歇运动对高脂膳食大鼠肠道炎症及α7型烟碱乙酰胆碱受体、核转录因子κΒ p65的影响[J].中华物理医学与康复杂志,2024,46(12):1072-1078
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中等强度持续运动与高强度间歇运动对高脂膳食大鼠肠道炎症及α7型烟碱乙酰胆碱受体、核转录因子κΒ p65的影响
  
DOI:10.3760/cma.j.cn421666-20240120-00076
中文关键词: 肠道炎症  核转录因子κΒ p65  肿瘤坏死因子α  α7型烟碱乙酰胆碱受体  高强度间歇运动  高脂膳食
英文关键词: Intestinal inflammation  Nuclear factor-κappa Bp65  Tumor necrosis factor-α  α7 nicotinic acetylcholine receptor  Interval training  High-fat diets  Training intensity
基金项目:河北省中央引导地方科技发展资金项目(246Z5701G)
作者单位
王伟欢 河北师范大学石家庄 050024
河北省人体运动生物信息测评重点实验室石家庄 050024 
代玉玺 河北师范大学石家庄 050024
河北省人体运动生物信息测评重点实验室石家庄 050024 
吴卫东 河北师范大学石家庄 050024
河南体育学院郑州 450044 
杜凝萃 河北师范大学石家庄 050024
河北省人体运动生物信息测评重点实验室石家庄 050024 
刘帅 河北师范大学石家庄 050024
河北省人体运动生物信息测评重点实验室石家庄 050024 
何玉秀 河北师范大学石家庄 050024
河北省人体运动生物信息测评重点实验室石家庄 050024 
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中文摘要:
      目的 观察12周的中等强度持续运动(MICT)与高强度间歇运动(HIIT)对高脂膳食大鼠肠道炎症及α7型烟碱乙酰胆碱受体(α7nAChR)、核转录因子κΒ p65(NF-κΒ p65)表达的影响。 方法 选取5周龄健康SD雄性大鼠32只,按照随机数字表法将其分为普通膳食安静组、高脂膳食安静组、高脂膳食MICT组、高脂膳食HIIT组,每组8只。高脂膳食MICT组以70%最大摄氧量(VO2max)的强度进行持续跑台运动,高脂膳食HIIT组以40%~45%VO2max和95%~99%VO2max的强度交替进行跑台运动,普通膳食安静组、高脂膳食安静组不进行运动干预。12周后,采用苏木精-伊红(HE)染色观察4组大鼠的肠道组织形态;测定大鼠血清的低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、甘油三酯(TG)、游离脂肪酸(FFA)含量;采用免疫荧光双标记和Western blot法检测大鼠肠道α7nAChR、NF-κΒ p65、肿瘤坏死因子α(TNF-α)的表达水平。 结果 与普通膳食安静组比较,高脂膳食安静组大鼠的终体重[(661.30±31.68)g]较重、总摄食量[(2011.84±77.40)g]较低,血清LDL[(65.50±20.65)mg/dL]、TG[(45.69±7.91)mg/dL]、FFA[(8.76±1.41)mg/dL]含量较高,HDL[(53.99±20.65)mg/dL]含量较低,肠道α7nAChR的蛋白表达量较低,NF-κΒ p65的平均荧光强度和蛋白表达量较高,TNF-α的蛋白表达量较高(P<0.05)。与高脂膳食安静组比较,高脂膳食MICT组、高脂膳食HIIT组的终体重较轻、总摄食量较低,LDL、FFA含量均较低,α7nAChR的平均荧光强度和蛋白表达量增加,NF-κΒ p65的平均荧光强度和的蛋白表达量降低,TNF-α的蛋白表达量较低(P<0.05)。与高脂膳食MICT组比较,高脂膳食HIIT组的HDL含量[(67.27±23.32)mg/dL]较高,NF-κΒ p65的平均荧光强度较低(P<0.05)。高脂膳食MICT组和高脂膳食HIIT组大鼠的结肠组织切片,表现为炎性浸润减轻,上皮损伤及黏膜隐窝破坏减少。 结论 12周的MICT和HIIT均可改善高脂膳食大鼠的肠道炎症状态,增加肠道α7nAChR蛋白表达,降低NF-κΒ p65、TNF-α表达。其中,HIIT的改善效果更好。
英文摘要:
      Objective To observe any effect of 12 weeks of moderate-intensity continuous training (MICT) and of high-intensity interval training (HIIT) on intestinal inflammation and the expression of α7 nicotinic acetylcholine receptor (α7nAChR) and nuclear factor kappa B p65 (NF-κΒp65) in rats fed a high-fat diet. Methods Thirty-two healthy 5-week-old male Sprague-Dawley rats were divided at random into a normal diet and quiet group, a high-fat diet and quiet group, a high-fat diet MICT group, and a high-fat diet HIIT group, each of 8. The high-fat diet MICT group underwent continuous treadmill exercise at 70% of their maximum oxygen uptake (VO2max), while the high-fat diet HIIT group did treadmill exercise at 40% to 45% of their VO2max alternating with intervals at 95% to 99% of their VO2max. The two quiet groups did no exercise. After 12 weeks, hematoxylin-eosin staining was used to observe the morphology of the rats′ intestinal tissue. Serum levels of low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG) and free fatty acids (FFA) were determined, and immunofluorescence double labeling and western blotting were applied to detect the expression levels of α7nAChR, NF-κB p65 and tumor necrosis factor α (TNF-α) in the rats′ intestines. Results Compared with the normal diet quiet group, the quiet group on a high fat diet had, on average, significantly heavier final body weights, lower total food intake, higher serum LDL, TG and FFA, lower HDL levels, and less protein expression of intestinal α 7nAChR. They showed higher average fluorescence intensity and expression of NF-κB p65 and TNF-α protein. Compared with the high-fat diet quiet group, the high-fat diet MICT and HIIT groups had significantly lighter final body weight, on average, lower total food intake, lower LDL and FFA levels and higher average fluorescence intensity. They showed significantly greater expression of α 7nAChR and NF-κB p65 protein, and lower expression of TNF-α protein. Compared with the high-fat diet MICT group, there was a significantly higher HDL content and lower average fluorescence intensity of NF-κ -Bp65 observed in the high-fat diet HIIT group. Moreover, reduced inflammatory infiltration, epithelial damage and mucosal crypt destruction were found in the colon tissue sections of both the high-fat diet MICT and HIIT groups. Conclusions Twelve weeks of either MICT or HIIT can relieve intestinal inflammation resulting from a high-fat diet, at least in rats. The training increases the intestinal expression of α 7nAChR protein, and reduces the expression of NF-κBp65 and TNF-α. HIIT is superior to MICT in its effects.
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