叶秋平,袁思,邓冰,等.电针廉泉穴和风府穴对吞咽相关脑区c-Fos特异性的影响[J].中华物理医学与康复杂志,2021,43(12):1087-1093
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| 电针廉泉穴和风府穴对吞咽相关脑区c-Fos特异性的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2021.12.008 |
| 中文关键词: 电针 吞咽相关脑区 廉泉穴 风府穴 |
| 英文关键词: Electroacupuncture Swallowing Lianquan acupoint Fengfu acupoint |
| 基金项目:国家自然科学金面上项目(81774406)；国家自然科学基金青年基金项目(82004469)；广东省普通高校重点实验室项目（2018KSYS006） ；广州中医药大学“双一流”与高水平大学学科协同创新团（2021XK01） |
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| 摘要点击次数: 1962 |
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| 中文摘要: |
| 目的 观察电针廉泉穴和风府穴对吞咽相关脑区（孤束核、臂旁核、初级运动皮质和初级感觉皮质）c-Fos的特异性的影响。 方法 选用C57小鼠20只，将其分为空白组（不做电针干预）、电针廉泉穴组（仅电针廉泉穴）、电针风府穴组（仅电针风府穴）和电针廉泉穴+风府穴组（电针风府穴和廉泉穴），每组5只小鼠。电针廉泉穴组、电针风府穴组和电针廉泉穴+风府穴组均接受对应穴位的电针治疗15 min，空白组不做电针干预。4组小鼠均于电针廉泉穴组、电针风府穴组和电针廉泉穴+风府穴组电针干预结束50 min后取材，进行免疫荧光染色，通过共聚焦显微镜观察并统计各个脑区（孤束核、臂旁核、初级运动皮质和初级感觉皮质）即刻早基因c-Fos的表达。 结果 在孤束核中，电针廉泉穴组的c-Fos阳性细胞数为（445.1±43.14）个/mm2高倍镜视野，显著高于电针风府穴组的（297.47±25.54）个/mm2高倍镜视野，差异有统计学意义（P＜0.001）。在臂旁核中，电针廉泉穴组、电针风府穴组和电针廉泉穴+风府穴组三组的c-Fos阳性细胞数组间差异均无统计学意义（P＞0.01）。在初级运动皮质中，电针风府穴组的c-Fos阳性细胞数与电针廉泉穴组和电针廉泉穴+风府穴组比较，差异均有统计学意义（P＜0.05）。在初级感觉皮质中，电针廉泉穴+风府穴组的c-Fos阳性细胞数比电针廉泉穴组和电针风府穴组高，差异均有统计学意义（P＜0.01）。 结论 在生理状态下，与电针风府穴、电针廉泉穴+风府穴相比，电针廉泉穴对孤束核的c-Fos激活更强；与电针廉泉穴、电针廉泉穴+风府穴相比，电针风府穴对初级运动皮质的c-Fos激活更强；与电针廉泉穴、电针风府穴相比，电针廉泉穴+风府穴对初级感觉皮质的c-Fos激活更强。 |
| 英文摘要: |
| Objective To observe any effects of electroacupuncture (EA) at acupoints CV23 and GV16 in activating c-Fos genes in the brain regions related to swallowing. Methods Twenty C57 mice were randomly divided into a blank group, an EA at CV23 group, an EA at GV16 group and an EA at CV23+GV16 group, each of 5. All except the mice in the blank group were given EA at the relevant acupoints CV23 or GV16 or both, respectively, for 15min. Samples were collected from all of the groups 50min after the EA treatment, and immunofluorescence staining was used to quantify the expression of the c-Fos gene in the brain regions controlling swallowing. Results In the nucleus tractus solitarii the average number of c-Fos positive cells of the CV23 group was (445.1±43.14)/mm2, significantly higher than the (297.47±25.54)/mm2 of the GV16 group. No significant differences were observed in the average number of c-Fos positive cells in parabrachial nucleus. In the primary motor cortex the average number of c-Fos positive cells in the GV16 group was significantly higher than in the other two groups. In the primary sensory cortex the CV23+GV16 group had a significantly higher average number. Conclusions EA at the CV23 most strongly activates the c-Fos gene in the nucleus tractus solitarii. For c-Fos activation in the primary motor cortex GV16 is the most effective point. For c-Fos activation in the primary sensory cortex, EA at CV23 and GV16 together is recommended. These results may guide the selection of acupoints in treating dysphagia. |
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