高玉海,王轩林,侯雪峰,等.LED多波段光谱治疗对大鼠股骨骨折愈合的影响[J].中华物理医学与康复杂志,2026,48(3):193-200
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| LED多波段光谱治疗对大鼠股骨骨折愈合的影响 |
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| DOI：10.3760/cma.j.cn421666-20250401-00288 |
| 中文关键词: 发光二极管 光生物调节 血管造影术 骨形成 骨折愈合 |
| 英文关键词: Light-emitting diodes Photobiomodulation Angiography Bone formation Fracture healing |
| 基金项目:中国人民解放军联勤保障部队第九四〇医院高层次人才计划项目 (2024-G3-10) |
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| 中文摘要: |
| 目的 探讨发光二极管 (LED) 多光谱治疗对大鼠股骨骨折愈合的影响。方法 选取 40 只雄性大鼠，用外科手术方法建立股骨中段开放骨折模型，采用随机数字表法将其分为对照组和治疗组，每组 20 只。将 2 组大鼠均置于治疗舱内，对照组仅模拟 LED 放置而不通电照射，治疗组采用 LED 多波段光谱照射。每 2 周对各组大鼠患肢行 X 线检查以观察骨痂形成情况；造模后第 4、8 周末，每组随机选取 3 只大鼠行患肢显微 CT 扫描，以观察骨折愈合情况，另取 3 只大鼠行患肢骨痂微血管造影，以观察血管生长情况；造模后第 1、2、4、8 周末，每组随机抽取 3 只大鼠行患肢 HE 染色；造模后第 1、2 周末，另取 3 只大鼠，提取患肢骨痂总蛋白，用免疫印迹法检测 COL-1、ALP、RUNX2、BMP-2、OSX 和 VEGF 蛋白的表达水平。结果 X 线及显微 CT 结果显示治疗组骨痂及骨折愈合速度显著优于对照组，骨折评分及骨体积的差异均具有统计学意义（P<0.05）；治疗组血管数量和贯通情况均优于对照组；HE 染色结果显示，2 组骨折愈合方式均以二期愈合为主，治疗组软骨细胞增殖钙化及骨小梁形成速度也显著优于对照组；免疫印迹法检测结果显示，LED 治疗后，COL-1、RUNX2、BMP-2、OSX、VEGF 和 ALP 的相对表达量均显著高于对照组（P<0.05）。结论 多波段光谱照射治疗可通过加速骨基质的形成及矿化促进骨折愈合，其作用机制可能与促进血管的生成及上调骨形成相关蛋白有关。 |
| 英文摘要: |
| Objective To document any effect of multi-spectral light on the healing of a femoral fracture using rats. Methods An open fracture model of mid-shaft fracture of the femur was established surgically in each of forty male rats. Then they were divided into a control model group (MC) and an irradiation therapy group (LEDT), with 20 rats in each group. Housed under identical conditions in treatment chambers, the LEDT group received active multi-spectral therapy from a light-emitting diode (LED), while the MC group underwent sham treatment (no light). Fracture healing was monitored by X-ray imaging of the affected limbs every two weeks. After 4 and 8 weeks, 6 rats per group were randomly allocated to either micro-CT analysis for fracture healing assessment or to microangiography for evaluation of vascularization. For histological examination, hematoxylin and eosin (HE) staining was performed on the affected limbs of 3 rats from the three groups 1, 2, 4 and 8 weeks after the modeling. One and two weeks after the modeling, callus tissue from another three rats per group was subjected to total protein extraction and western blotting analysis to quantify the expression of COL-1、ALP、RUNX2、BMP-2、OSX, and VEGF protein. Results The radiographic and micro-CT assessments demonstrated significantly enhanced callus formation and faster fracture healing in the LEDT group compared to the MC group, with significant differences in fracture scores and bone volume. The microangiography revealed superior vascular density and patency in the LEDT group, and the HE staining confirmed that secondary healing was the primary mode of repair in all cases. The LEDT group, though, exhibited accelerated chondrocyte proliferation, calcification, and trabecular bone formation compared to the control group. Additionally, the western blotting consistently showed at each time point that the expression of COL-1、RUNX2、BMP-2、OSX、VEGF and ALP proteins had been significantly upregulated in the LEDT group compared to the control group. Conclusions Multi-spectral LED irradiation promotes fracture healing by enhancing bone matrix formation and mineralization. The mechanism may involve the stimulation of angiogenesis and the upregulation of osteogenic protein expression. |
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