金军辉,程红宇,梁英姿,等.高强度间歇训练对自发性高血压大鼠心脏重塑的影响[J].中华物理医学与康复杂志,2026,48(1):1-7
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| 高强度间歇训练对自发性高血压大鼠心脏重塑的影响 |
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| DOI：10.3760/cma.j.cn421666-20250427-00369 |
| 中文关键词: 高强度间歇训练 自发性高血压大鼠 心脏重塑 心脏肥大 心肌纤维化 |
| 英文关键词: Interval training Spontaneous hypertension Cardiac remodeling Cardiac hypertrophy Myocardial fibrosis |
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| 中文摘要: |
| 目的 观察高强度间歇性训练（HIIT）对高血压大鼠心脏重塑的影响，并探讨丝裂原活化蛋白激酶（MAPK）信号途径在其间的作用机制。 方法 采用随机数字表法将30只自发性高血压大鼠分为对照组和运动组，每组15只，同时选取15只Wistar Kyoto大鼠纳入健康组。健康组及对照组大鼠均安静饲养，运动组大鼠则给予12周跑台HIIT训练。于末次干预结束后48 h，采用递增负荷跑台运动实验检测各组大鼠运动能力，采用尾动脉套管法检测血压水平，采用超声心动图评估心脏结构与功能，通过分离乳头肌检测离体心肌收缩力，采用HE染色或Masson染色对心肌组织进行病理学观察，并计算心肌细胞横截面积及胶原容积分数，采用免疫印迹法检测各组大鼠心脏MAPK及其下游转录因子蛋白表达量。 结果 与健康组比较，对照组大鼠血压升高（P<0.05），运动能力下降（P<0.05），左心室舒张末期内径（LVDD）、左心室后壁厚度（PWT）、乳头肌静息张力（RT）增加（P<0.05），左心室缩短分数（LVFS）、左心室射血分数（LVEF）、E/A比值下降（P<0.05），心肌细胞横截面积及胶原容积分数增加（P<0.05），磷酸化-激活转录因子2/激活转录因子2（p-ATF2/ATF2）比值上调（P<0.05）。与对照组比较，运动组大鼠血压降低（P<0.05），运动能力改善（P<0.05），LVDD、PWT、LVEF、LVFS、E/A比值增加（P<0.05），乳头肌RT下降（P<0.05），心肌细胞横截面积增加（P<0.05），胶原容积分数下降（P<0.05），p-ATF2/ATF2比值下调（P<0.05）。 结论 长期HIIT干预能改善高血压大鼠心脏重塑，其作用机制可能与抑制ATF2磷酸化激活有关。 |
| 英文摘要: |
| Objective To observe any impact of high-intensity interval training (HIIT) on cardiac remodeling in hypertensive rats, and to explore any role of the mitogen-activated protein kinase (MAPK) signaling pathway. Methods Thirty spontaneously hypertensive rats were randomly divided into a control group and an exercise group (each of 15), while another 15 Wistar Kyoto rats composed a healthy group. The healthy and control groups were kept sedentary, while the exercise group underwent 12 weeks of HIIT on a treadmill. Forty-eight hours after the last exercise session, an incremental load treadmill exercise test was conducted to assess each rat′s exercise capacity, while the tail-cuff method was used to measure blood pressure. Echocardiography was performed to evaluate cardiac structure and function, and isolated papillary muscle contraction was measured. Histopathological observations of the myocardium used hematoxylin & eosin or Masson staining to obtain cardiomyocyte cross-sectional areas and collagen volume fractions. The expression of cardiac MAPK and its downstream transcription factor proteins were detected using western blotting. Results Compared with the healthy group, the control group on average exhibited significantly elevated blood pressure, significantly larger end-diastolic diameter of the left ventricle (LVDD), significantly greater posterior wall thickness (PWT), and significantly greater resting papillary muscle tension (RT). On average the control group demonstrated significantly less exercise capacity, left ventricular fractional shortening (LVFS), a significantly smaller left ventricle ejection fraction (LVEF), and significantly smaller E/A ratios. Additionally, the cardiomyocyte cross-sectional areas and collagen volume fractions were significantly greater on average, and the ratio of phosphorylated-activating transcription factor 2 to activating transcription factor 2 (the p-ATF2/ATF2 ratio) tended to be significantly greater than among the healthy group. Compared with the control group, the exercise group exhibited significantly lower average blood pressure, significantly less papillary muscle RT, and significantly smaller collagen volume fractions and p-ATF2/ATF2 ratios. On average the exercise group also had significantly increased exercise capacity, significantly better LVDD, PWT, LVEF, LVFS and E/A ratio results and greater cardiomyocyte cross-sectional areas. Conclusions Long-term HIIT can improve cardiac remodeling in hypertensive rats, and the mechanism may be related to the inhibition of ATF2 phosphorylation and activation. |
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