吴金涛,孙勇,梁英姿,等.有氧运动对2型糖尿病大鼠内皮祖细胞功能及Akt/eNOS信号通路的影响[J].中华物理医学与康复杂志,2025,47(7):595-600
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| 有氧运动对2型糖尿病大鼠内皮祖细胞功能及Akt/eNOS信号通路的影响 |
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| DOI：10.3760/cma.j.cn421666-20250122-00069 |
| 中文关键词: 有氧运动 2型糖尿病 内皮祖细胞 管腔形成 信号通路 |
| 英文关键词: Aerobic exercise Type 2 diabetes Endothelial progenitor cells Lumen formation Signaling pathways |
| 基金项目:河南省科技攻关项目（252102321132） |
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| 中文摘要: |
| 目的 探讨有氧运动对2型糖尿病大鼠内皮祖细胞（EPCs）功能及Akt/eNOS信号通路的影响。方法 选取45只6周龄无特定病原体级（SPF）雄性SD大鼠，将其随机分为正常组、模型组及运动组。通过高脂饲料喂养、脲佐菌素注射将模型组及运动组大鼠制成2型糖尿病动物模型，运动组大鼠于制模成功后给予8周无负重游泳训练。待8周游泳训练结束后，通过腹主动脉取血检测各组大鼠外周血中EPCs数量以及血清一氧化氮（NO）、血管内皮生长因子（VEGF）含量，取股骨及胫骨分离培养骨髓EPCs，采用Matrigel管腔形成实验检测各组大鼠骨髓EPCs的成管能力，采用Western blot法检测各组大鼠EPCs中蛋白激酶B（Akt）及内皮型一氧化氮合酶（eNOS）蛋白表达量。 结果 与正常组比较，模型组外周血中EPCs计数及血清NO、VEGF含量均显著下降（P<0.05），骨髓EPCs成管能力明显减弱（P<0.05），磷酸化Akt（p-Akt）和p-eNOS蛋白表达均显著下调（P<0.05）。与模型组比较，运动组EPCs数量明显增加（P<0.05），血清中NO及VEGF含量显著升高（P<0.05），骨髓EPCs成管能力明显改善（P<0.05），p-Akt及p-eNOS蛋白表达均显著上调（P<0.05）。 结论 有氧运动能改善糖尿病大鼠EPCs功能，其作用机制可能与调控Akt/eNOS信号通路有关。 |
| 英文摘要: |
| Objective To investigate any effect of aerobic exercise on the functioning of endothelial progenitor cells (EPCs) and on the Akt/eNOS signaling pathway in type 2 diabetes. Methods Forty-five 6-week-old Sprague-Dawley rats free of specific pathogens were randomly divided into a normal control group, a diabetic model group and an exercise group. Type 2 diabetes mellitus was induced in the model and exercise groups by feeding a high-fat diet and streptozotocin injection. After successful modeling, the exercise group underwent 8 weeks of non-weight-bearing swimming training after which blood was collected from their abdominal aortas to measure EPCs, serum nitric oxide and the level of vascular endothelial growth factor (VEGF). Bone marrow-derived EPCs were isolated from the rats′ femurs and tibias for in vitro culture. The cells′ tube formation capacity was assessed using Matrigel assays, while the expression of protein kinase B (Akt) and endothelial nitric oxide synthase (eNOS) were determined using western blotting. Results Compared with the normal group, the model group exhibited significantly reduced counts of EPCs in their peripheral blood. Serum NO and VEGF were also significantly lower, on average, and tube formation capacity was significantly impaired. p-Akt and p-eNOS protein expression were significantly downregulated. In contrast, the exercise group showed significantly increased EPC counts, elevated serum NO and VEGF levels, improved tube formation, and upregulated p-Akt and p-eNOS expression compared with the model group. Conclusions Aerobic exercise improves EPC functioning in diabetic rats, and its mechanism may be associated with the regulation of the Akt/eNOS signaling pathway. |
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