王敏飞,王倩倩,白春宏,等.miR-122在高强度间歇训练改善糖尿病大鼠非酒精性脂肪性肝病中的作用及机制探讨[J].中华物理医学与康复杂志,2025,47(1):7-12
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| miR-122在高强度间歇训练改善糖尿病大鼠非酒精性脂肪性肝病中的作用及机制探讨 |
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| DOI：10.3760/cma.j.cn421666-20240828-00699 |
| 中文关键词: 高强度间歇训练 中等强度持续训练 miR-122 糖尿病 非酒精性脂肪性肝病 |
| 英文关键词: Interval training Training intensity miR-122 Diabetes Fatty liver disease |
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| 中文摘要: |
| 目的 观察高强度间歇训练（HIIT）对糖尿病大鼠非酒精性脂肪性肝病（NAFLD）的疗效，并探讨miR-122在其治疗机制中的作用。 方法 采用随机数字表法将40只Wistar大鼠分为健康对照组、安静组、高强度间歇训练组(HIIT组)和中等强度持续训练组（MICT组）。通过高脂高果糖饮食将安静组、HIIT组及MICT组大鼠制成2型糖尿病伴NAFLD动物模型。制模后健康对照组及安静组大鼠均置于鼠笼内安静饲养，HIIT组及MICT组大鼠则分别给予相应强度的跑台运动，连续训练8周。于末次训练后48 h取各组大鼠血浆检测生化标志物，取肝脏组织进行苏木精-伊红（HE）染色及组织学观察，采用实时荧光定量PCR技术检测miR-122表达，采用免疫印迹法检测脂肪酸合酶（FAS）、乙酰辅酶A羧基酶（ACC）、固醇调节元件结合蛋白1c（SREBP1c）及过氧化物酶体增殖物激活受体γ（PPARγ）蛋白表达。 结果 与健康对照组比较，安静组空腹血糖（FBG）、甘油三酯（TG）、总胆固醇（TC）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、胰岛素（Ins）及胰岛素抵抗指数（HOMA-IR）均显著升高（P<0.05）；与安静组比较，HIIT组和MICT组FBG、TG、TC、Ins及HOMA-IR均明显降低（P<0.05），HIIT组ALT及AST也明显降低（P<0.05），并且HIIT组TG、TC、ALT及AST亦显著低于MICT组水平（P<0.05）。与安静组比较，HIIT组及MICT组肝脏脂肪变性评分均明显降低（P<0.05）；并且HIIT组脂肪变性评分亦显著低于MICT组水平（P<0.05）。与健康对照组比较，安静组miR-122表达明显降低（P<0.05），SREBP1c、PPARγ、FAS及ACC表达显著升高（P<0.05）；与安静组比较，HIIT组和MICT组miR-122表达明显升高（P<0.05），SREBP1c、PPARγ、FAS及ACC表达则显著降低（P<0.05）；并且HIIT组miR-122表达亦高于MICT组水平（P<0.05），SREBP1c、PPARγ、FAS及ACC表达较MICT组显著降低（P<0.05）。 结论 MICT及HIIT训练均可改善糖尿病大鼠NAFLD，并以HIIT训练的治疗效果更佳，其治疗机制可能与增强肝脏组织中miR-122表达有关。 |
| 英文摘要: |
| Objective To observe the effect of high-intensity interval training (HIIT) on diabetic rats with non-alcoholic fatty liver disease (NAFLD), and to explore the role of miR-122 in it. Methods Forty Wistar rats were randomly divided into a healthy control group, a sedentary group, a high-intensity interval training (HIIT) group and a moderate-intensity continuous training (MICT) group using a random number table. All except the healthy control group had type 2 diabetes with NAFLD induced using a high-fat and high-fructose diet. The healthy control and sedentary groups were kept quiet in their cages, while the HIIT and MICT groups performed treadmill exercise of the corresponding intensity for 8 consecutive weeks. At 48h after the last training, plasma was collected to measure biochemical markers, and the livers were resected for histological observation using hematoxylin and eosin staining. miR-122 expression was measured using real-time fluorescent quantitative polymerase chain reactions, while the protein expressions of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1c (SREBP1c) and peroxisome proliferator-activated receptor γ (PPARγ) were detected using western blotting. Results Compared with the healthy control group, the average fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), insulin and insulin resistance index (HOMA-IR) had increased significantly in the sedentary group. Compared with the sedentary group, the average FBG, TG, TC, insulin and HOMA-IR had decreased significantly in both the HIIT and MICT groups, and the average ALT and AST in the HIIT group had also decreased significantly. The average TG, TC, ALT and AST levels in the HIIT group were then significantly lower than in the MICT group. Compared with the sedentary group, the average liver steatosis score had decreased significantly in both the HIIT and MICT groups, with that of the HIIT group significantly lower than in the MICT group, on average. Compared with the healthy control group, the expression of miR-122 had decreased significantly, but that of SREBP1c, PPARγ, FAS and ACC had increased significantly in the sedentary group. And compared with the sedentary group, the expression of miR-122 had increased significantly in both the HIIT and MICT groups, on average, but that of SREBP1c, PPARγ, FAS and ACC had decreased significantly. Average miR-122 levels in the HIIT group were then significantly higher, and those of SREBP1c, PPARγ, FAS and ACC were significantly lower than in the MICT group. Conclusions Either MICT or HIIT training can relieve NAFLD in rats modeling diabetes, but HIIT has a better therapeutic effect. Its therapeutic mechanism may be related to the upregulation of miR-122 in the liver. |
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