吴洁,乔爱弟,胡胜艳,等.电针百会穴和大椎穴对脑瘫大鼠学习记忆能力及miR-126介导的血管内皮生长因子/Notch1通路的影响[J].中华物理医学与康复杂志,2024,46(8):673-679
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| 电针百会穴和大椎穴对脑瘫大鼠学习记忆能力及miR-126介导的血管内皮生长因子/Notch1通路的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2024.08.001 |
| 中文关键词: 脑瘫 电针 学习 记忆 miR-126 血管内皮生长因子/Notch1通路 |
| 英文关键词: Cerebral palsy Electroacupuncture Learning Memory miR-126 VEGF/Notch1 signalling |
| 基金项目:浙江省基础公益研究计划项目（LGF22H270008）；金华市公益性技术应用研究项目（2022-4-350） |
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| 中文摘要: |
| 目的 探讨电针百会穴和大椎穴对脑瘫大鼠学习记忆能力及miR-126介导的血管内皮生长因子（VEGF）/Notch1通路的影响。 方法 选取健康7日龄SD雄性大鼠54只，按照随机数字表法将其分为假手术组、模型组、电针组，每组18只。模型组、电针组采用左颈总动脉（LCCA）结扎结合缺氧的方法制备脑瘫大鼠模型。造模后24 h，电针组大鼠予以百会穴和大椎穴电针干预，共14次，隔日1次，持续至第28天。造模后第29天，采用Morris水迷宫法检测3组大鼠的学习记忆能力。采用免疫组化染色法观察大鼠海马组织VEGF及血管内皮细胞生长因子受体-2（VEGFR-2）的表达量。采用Western blot法检测大鼠海马组织Notch1、发状分裂相关增强子1(Hes1)蛋白水平。采用RT-qPCR法检测大鼠海马组织miR-126的表达水平。 结果 与假手术组比较，模型组大鼠的学习记忆能力明显降低（P<0.05），miR-126的表达水平下调，VEGF、VEGFR-2的表达水平上调，Notch1、Hes1蛋白表达水平上调（P<0.05）；与模型组比较，电针组大鼠的学习记忆能力明显改善（P<0.05），miR-126的表达水平上调，VEGF、VEGFR-2的表达水平上调，Notch1、Hes1蛋白表达水平上调（P<0.05）。 结论 电针百会穴和大椎穴可以改善脑瘫大鼠的学习记忆能力，其机制可能与miR-126调控VEGF/Notch1通路，进而促进血管新生有关。 |
| 英文摘要: |
| Objective To explore any effect of electroacupuncture (EA) at the Baihui (DU20) and Dazhui (GV14) acupoints on the learning and memory of rats modeling cerebral palsy (CP), and on the miR-126 regulated VEGF/Notch1 pathway. Methods Fifty-four male Sprague-Dawley rats 7 days old with an average weight of (20±5g) were randomly divided into a sham operation group, a model group, and an electroacupuncture group, each of 18. All except the sham operation group had a model of CP induced using left common carotid artery ligation combined with hypoxia. Twenty-four hours after successful modelling, the EA group received 14 sessions of electroacupuncture at the DU20 and GV14 acupoints, once every other day. On the 29th day, learning and memory ability were evaluated using a Morris Water Maze. The expression of vascular endothelial growth factors (VEGF and VEGFR-2) was detected using immunohistochemical staining. The levels of notch1 and hes1 protein were quantified using western blotting, and the expression of miR-126 RNA was quantified using reverse transcription polymerase chain reactions (RT-qPCRs). Results Compared with the sham group, there was a significant decrease in the average learning and memory, and the level of miR126 in the model group, but a significant increase in the expression of VEGF and VEGFR-2 and the levels of notch1 and hes1. Significant improvement was observed in all of the measurements of the EA group compared with the model group. Conclusions Electroacupuncture at the DU20 and GV14 acupoints can improve the ability of learning and memory of rats modeling CP. The mechanism may be related to regulation of the VEGF/notch1 pathway by miR-126 and its promotion of angiogenesis. |
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