吴金涛,万琼,刘静,等.高强度运动对糖尿病心肌病大鼠心脏重塑及亚硝基-氧化还原失衡的影响[J].中华物理医学与康复杂志,2024,46(6):500-505
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| 高强度运动对糖尿病心肌病大鼠心脏重塑及亚硝基-氧化还原失衡的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2024.06.003 |
| 中文关键词: 高强度运动 糖尿病心肌病 心脏重塑 氧化应激 亚硝基-氧化还原平衡 |
| 英文关键词: High-intensity training Diabetic cardiomyopathy Cardiac remodeling Oxidative stress Nitroso-redox balance |
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| 中文摘要: |
| 目的 观察高强度运动对1型糖尿病大鼠心脏重塑及亚硝基-氧化还原失衡的影响，为制订糖尿病心肌病患者运动康复处方提供理论依据及干预靶点。 方法 从45只8周龄健康雄性SD大鼠中取10只纳入正常对照组（NC组），剩余大鼠通过注射链脲佐菌素方式制作1型糖尿病大鼠模型，并随机分为糖尿病对照组（DMC组）及糖尿病运动组（DME组）。NC组及DMC组大鼠均置于鼠笼内安静饲养，DME组大鼠则给予4周高强度跑台运动干预（5次/周）。于末次训练结束48 h后利用超声成像系统检测各组大鼠心脏结构及功能，采用葡萄糖氧化酶法测定空腹血糖含量；取各组大鼠心肌组织进行HE染色和天狼星红染色，经病理学观察后分析对比各组大鼠心肌细胞横断面积（CSA）及间质胶原容积分数（CVF），采用高效液相色谱法测定心肌四氢生物喋呤（BH4）含量，采用Western blot法检测心肌p47phox和p67phox、内皮型一氧化氮合酶（eNOS）、eNOS二聚体及单体蛋白表达量。 结果 与NC组比较，DMC组血糖、左心室舒张末期内径（LVEDD）、左心室收缩末期内径（LVESD）、心肌CSA及CVF、p47phox和p67phox蛋白表达量均显著增加（P<0.05），eNOS二聚体/单体比值及BH4含量则显著降低（P<0.05）；与DMC组比较，DME组血糖、LVEDD、LVESD、心肌CSA及CVF均显著降低（P<0.05）；p47phox及p67phox蛋白表达量、eNOS二聚体/单体比值和BH4含量则无显著差异（P>0.05）。 结论 短期高强度运动能抑制糖尿病心肌病大鼠心脏重塑进程，但对大鼠体内亚硝基氧化-还原失衡无明显改善作用。 |
| 英文摘要: |
| Objective To observe any effect of high-intensity exercise on cardiac remodeling and nitroso-redox imbalance in rats modeling type 1 diabetes so as to provide a theoretical basis and targets for prescribing rehabilitation exercise for patients with diabetic cardiomyopathy. Methods Forty-five 8-week-old healthy, male Sprague-Dawley rats were randomly divided into a normal control group (NC) of 10, a diabetic control group (DMC) and a diabetic exercise group (DME). Type 1 diabetes was induced in the DMC and DME groups by injecting streptozotocin. The NC and DMC group rats were then fed quietly in their cages, while the DME group rats performed high-intensity treadmill exercise 5 times a week for 4 weeks. Forty-eight hours after the last training, cardiac structure and function were detected by echocardiography, and fasting blood glucose was measured by the glucose oxidase method. Cardiomyocyte cross-sectional area (CSA) and interstitial collagen volume fraction (CVF) were observed. Myocardial tetrahydrobiopterin (BH4) content was determined chromatographically, and myocardial p47phox and p67phox protein, endothelial nitric oxide synthase (eNOS), and eNOS dimer and monomer protein levels were detected using western blotting. Results Compared with the NC group, the average blood glucose, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), myocardial CSA and CVF, and p47phox and p67phox protein expression had all increased significantly, but the eNOS dimer/monomer ratio and BH4 content had decreased significantly in the DMC group. In the DME group the average blood glucose, LVEDD, LVESD, myocardial CSA and CVF were significantly lower than in the DMC group, while there was no significant difference between the two groups in p47phox or p67phox protein expression, the eNOS dimer/monomer ratio or BH4 content. Conclusion Short-term, high-intensity exercise can inhibit cardiac remodeling in rats with diabetic cardiomyopathy, but it does not relieve the nitro-redox imbalance. |
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