寇冠,万震,刘晓哲,等.有氧运动对自发性高血压大鼠心肌内皮型一氧化氮合酶解耦联的影响[J].中华物理医学与康复杂志,2024,46(3):193-198
扫码阅读全文
|
| 有氧运动对自发性高血压大鼠心肌内皮型一氧化氮合酶解耦联的影响 |
|
| |
| DOI：10.3760/cma.j.issn.0254-1424.2024.03.001 |
| 中文关键词: 有氧运动 高血压 心脏重塑 内皮型一氧化氮合酶 解耦联 |
| 英文关键词: Aerobic exercise Hypertension Cardiac remodeling Endothelium Nitric oxide synthase |
| 基金项目: |
|
| 摘要点击次数: 412 |
| 全文下载次数: 358 |
| 中文摘要: |
| 目的 观察规律有氧运动对自发性高血压大鼠（SHR）心脏重塑的影响，并探讨内皮型一氧化氮合酶（eNOS）解耦联在其间的作用机制。 方法 采用随机数字表法将30只6周龄健康雄性SHR大鼠分为安静组和运动组，每组15只；同时选取10只鼠龄、性别相匹配的Wistar-Kyoto大鼠纳入正常对照组。将正常对照组和安静组大鼠置于鼠笼内安静饲养，运动组大鼠则给予8周中等强度跑台运动干预，每周运动5次。于末次训练结束48 h后采用超声心动图设备检测各组大鼠心脏结构及功能，分别对其心肌组织进行麦胚凝集素和天狼星红染色，观察病理学改变并获取心肌细胞横截面积（CSA）及间质胶原容积分数（CVF），采用TUNEL染色法检测心肌细胞凋亡率，采用高效液相色谱法测定心肌四氢生物喋呤（BH4）含量，采用Western blot法检测心肌组织eNOS总蛋白、eNOS二聚体及单体蛋白表达量。 结果 与正常对照组比较，安静组左心室壁厚度（LVWT）、心肌CSA和CVF升高（P＜0.05），左心室舒张末期内径（LVEDD）和左心室射血分数（LVEF）降低，心肌细胞凋亡率增加（P＜0.05），eNOS单体上调（P＜0.05），eNOS二聚体、eNOS二聚体/单体比值以及心肌BH4含量下降（P＜0.05）；与安静组比较，运动组心肌CVF降低（P＜0.05），LVEDD和LVEF升高（P＜0.05），心肌细胞凋亡率下降（P＜0.05），eNOS单体下调（P＜0.05），eNOS二聚体、eNOS二聚体/单体比值以及心肌BH4含量增加（P＜0.05），LVWT和CSA组间均无显著差异（P＞0.05）。3组大鼠eNOS总蛋白含量组间均无明显差异（P＞0.05）。 结论 规律有氧运动可能通过调控eNOS解耦联改善SHR大鼠心脏重塑。 |
| 英文摘要: |
| Objective To observe any effect of regular aerobic exercise on cardiac remodeling in spontaneously hypertensive rats (SHR) and explore the mechanism of endothelial nitric oxide synthase (eNOS) uncoupling. Methods Thirty 6-week-old healthy male SHR were divided into a sedentary group and an exercise group, each of 15. Another ten age- and sex-matched Wistar-Kyoto rats were set as a normal control group. The animals in the normal control and sedentary groups were fed quietly in their cages, and those in the exercise group performed moderate intensity treadmill exercise for 8 weeks (5 times per week). Forty-eight hours after the last training, echocardiography was applied to document cardiac structure and function in both groups. Wheat germ agglutinin staining and Picrosirius Red staining were used to obtain the cardiomyocyte cross sectional areas (CSAs) and interstitial collagen volume fractions (CVFs) of all of the mice. The rates of cardiomyocyte apoptosis were measured using TUNEL staining, and myocardial tetrahydrobiopterin (BH4) content was measured using high-performance liquid chromatography. Total eNOS, eNOS dimer and eNOS monomer protein expression in the myocardia were detected using western blotting. Results Compared with the normal control group, the left ventricular wall thickness (LVWT), myocardial CSA, CVF, apoptosis of cardiomyocytes and eNOS monomer levels were significantly higher in the sedentary group, on average. But the end-diastolic diameter (LVEDD) and ejection fraction (LVEF) of the left ventricle and the levels of eNOS dimer and myocardial BH4 and the eNOS dimer/monomer ratio tended to be lower. Comparing the exercise group with the sedentary group, the average LVEDD, LVEF, eNOS dimer, eNOS dimer/monomer ratio and myocardial BH4 content were significantly higher in the exercise group, but the myocardial CVF, cardiomyocyte apoptosis and eNOS monomer levels were significantly lower. LVWT and CSA were not significantly different. There were no significant differences in the total eNOS protein levels among the three groups. Conclusion Regular aerobic exercise might improve cardiac remodeling in cases of spontaneous hypertension regulating eNOS uncoupling, at least in rats. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
