林爱金,王乃针,郑军凡,等.骨髓间充质干细胞治疗新生鼠缺氧缺血性脑损伤的研究[J].中华物理医学与康复杂志,2023,45(7):585-591
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| 骨髓间充质干细胞治疗新生鼠缺氧缺血性脑损伤的研究 |
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| DOI：10.3760/cma.j.issn.0254-1424.2023.07.002 |
| 中文关键词: 骨髓间充质干细胞 缺氧缺血性脑损伤 小胶质细胞 神经元 |
| 英文关键词: Bone marrow Mesenchymal stem cells Hypoxia Ischemia Brain damage Microglia Neurons |
| 基金项目:福建省创伤骨科急救与康复临床医学研究中心（2020Y2014）;云南省科技厅科技计划项目基础研究专项（202301AT070263）；昆明医科大学研究生创新基金（2022B20） |
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| 中文摘要: |
| 目的 观察骨髓间充质干细胞移植治疗新生鼠缺氧缺血性脑损伤对小胶质细胞和神经元表达的影响。 方法 将10日龄C57BL/6小鼠60只按随机区组法分为假手术组、缺氧缺血组、安慰剂组和干细胞组，每组15只小鼠。缺氧缺血组、安慰剂组和干细胞组均进行缺氧缺血模型制备，假手术组仅颈部切口后缝合。安慰剂组造模完成后用脑立体定位仪下在前囟点注射磷酸盐缓冲液，干细胞组造模完成后用同样的方法在同一位置注射骨髓间充质干细胞。干细胞组移植7 d后，取4组小鼠的脑组织，用透射电镜观察其脑组织超微结构，采用免疫荧光染色观察4组小鼠左侧脑皮质神经元和小胶质细胞表达情况，并进行比较。 结果 干细胞移植7 d后，干细胞组神经元形态改善，神经纤维肿胀减轻。干细胞移植7 d后，皮质区神经元的表达存在组间差异［F(3,8)=88.080,P<0.05］，干细胞组小鼠左侧皮质神经元表达显著多于缺氧缺血组和安慰剂组，差异均有统计学意义（P<0.05）；干细胞移植7 d后，皮质区小胶质细胞的表达存在组间差异［F(3,8)=11.331,P=0.003］，干细胞组小胶质细胞的表达显著低于缺氧缺血组和安慰剂组，差异均有统计学意义（P<0.05）。 结论 骨髓间充质干细胞治疗新生鼠缺氧缺血性脑损伤可能通过抑制小胶质细胞表达来诱导神经元再生，减轻炎症反应。 |
| 英文摘要: |
| Objective To observe any effect of transplanting bone marrow mesenchymal stem cells (BMSCs) on microglia and neuron expression in newborn mice with hypoxic-ischemic brain damage (HIBD). Methods Sixty 10-day-old C57BL/6 mice were randomly divided into a sham operation group, a hypoxic-ischemia group, a placebo group and a stem cell group, each of 15. The hypoxia-ischemia model was induced in the hypoxia-ischemia, placebo and stem cell groups, while the sham operation group was sutured after the neck incision. After successful modeling, the rats in the stem cell group were injected with BMSCs into the bregma while those in the placebo group received phosphate buffered saline. Seven days later, brain tissue was resected and its structure was observed using transmission electron microscopy. Immunofluorescence staining was performed to observe the expression of microglia and neurons in the left cerebral cortex. Results Seven days after stem cell transplantation, the neuron morphology had improved and nerve fiber swelling was relieved in the stem cell group. The average expression of neurons was significantly greater in the stem cell group compared with the hypoxic-ischemia and placebo groups, while the expression of microglia was significantly lower. Conclusions Bone marrow mesenchymal stem cells may induce neuron regeneration and reduce inflammatory response by inhibiting the expression of microglia, at least in neonatal rats modeling hypoxic-ischemic brain injury. |
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