李响,陈运峰,邵士光,等.环境富集对坐骨神经挤压伤模型小鼠神经再生的影响[J].中华物理医学与康复杂志,2023,45(2):103-108
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| 环境富集对坐骨神经挤压伤模型小鼠神经再生的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2023.02.002 |
| 中文关键词: 坐骨神经 神经再生 环境富集 |
| 英文关键词: Sciatic nerve Nerve regeneration Schwann cells Environmental enrichment |
| 基金项目:济宁市重点研发计划项目（2020YXNS025，2021YXNS110）；国家自然科学基金（82101420） |
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| 中文摘要: |
| 目的 探讨环境富集对坐骨神经挤压伤模型小鼠神经再生的作用及机制。 方法 首先对22只C57BL/6小鼠进行坐骨神经挤压建立动物模型，随后按随机数字表法将模型小鼠分为干预组和对照组，干预组小鼠置于具备环境富集的鼠笼内进行干预，对照组置于标准鼠笼中饲养。造模成功2周后，2组小鼠均行坐骨神经电生理检查和步态分析，并应用甲苯胺蓝染色观察坐骨神经有髓神经纤维的比例，采用免疫荧光测定2组坐骨神经中的生长相关蛋白43（GAP43）、髓鞘碱性蛋白（MBP）以及p75神经营养素受体（p75NTR）表达的差异。 结果 ①坐骨神经电生理检测显示，干预组小鼠坐骨神经复合肌肉动作电位（CMAP)的潜伏期［（1.05±0.04）ms］较对照组［（1.98±0.30）ms］明显缩短(P<0.05)，而其波幅［干预组(10.63±0.90)mV］则明显高于对照组［（6.58±1.25）mV］，且组间差异有统计学意义（P<0.05）；②小鼠步态分析显示，干预组小鼠的平均接触强度［(160.60±20.45)AU］、步幅［(5.11±0.58)cm］和步速［(53.06±7.20)cm/s］ 均较对照组［（122.70±15.04）AU、（4.00±0.61）cm、（39.38±9.61）cm/s］有明显增加（P<0.05），而其步轴角［（21.88±2.13）°］则较对照组［(30.74±5.93)°］明显减小（P<0.05）；③经甲苯胺蓝染色镜下观，干预组神经纤维排列相对整齐、密集，有髓纤维数量较对照组明显增多（P<0.05）；④免疫荧光染色定量分析显示，干预组坐骨神经中的MBP、GAP43、p75NTR水平均较对照组明显提高（P<0.05）。 结论 环境富集可通过促进施万细胞的增殖、形成髓鞘而促进坐骨神经挤压伤小鼠模型中损伤神经的再生和功能的恢复。 |
| 英文摘要: |
| Objective To document any effect of environmental enrichment on nerve regeneration in a mouse model of sciatic nerve compression and explore its mechanism. Methods A crushed sciatic nerve model was successfully established in 22 C57BL/6 mice, and they were then randomly divided into an intervention group and a control group. The mice of the intervention group were raised in a cage with an enriched environment, while those of the control group were kept in a standard cage. Two weeks later, both groups′ gait was analyzed and the compound muscle action potential (CMAP) of the sciatic nerve was measured. The proportion of myelinated sciatic nerve fibers was examined using toluidine blue staining, and the expression of myelin basic protein (MBP), growth associated protein-43 (GAP43) and p75 neurotrophin receptor (p75NTR) was measured using immunofluorescence intensity. Results ①The latency of the CMAP ［(1.05±0.04)ms］ was significantly shortened in the intervention group compared with the control group and the amplitude was significantly higher. ②Gait analysis showed a significant increase in the average contact intensity, stride length and stride rate of the intervention group compared with the control group. However, the step axis angle of the intervention group was significantly smaller than in the control group on average. ③The stained nerve fibers in the intervention group were orderly and dense, and the average number of myelinated fibers was significantly greater than in the control group. ④Quantitative analysis of the immunofluorescence showed that the levels of MBP, GAP43 and p75NTR in the sciatic nerves of the intervention group were, on average, significantly higher than in the control group. Conclusion An enriched environmental can promote the regeneration and functional recovery of crushed sciatic nerves by promoting the proliferation and myelination of Schwann cells. |
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