朱明强,赵东帅,丁冲.重复磁刺激对人神经母细胞瘤细胞系SH-SY5Y细胞生长和分化的影响[J].中华物理医学与康复杂志,2022,44(9):769-773
扫码阅读全文
|
| 重复磁刺激对人神经母细胞瘤细胞系SH-SY5Y细胞生长和分化的影响 |
|
| |
| DOI：10.3760/cma.j.issn.0254-1424.2022.09.001 |
| 中文关键词: 重复磁刺激 SH-SY5Y细胞 细胞增殖 细胞凋亡 细胞分化 |
| 英文关键词: Repeated magnetic stimulation SH-SY5Y cells Cell proliferation Cell apoptosis Cell differentiation |
| 基金项目:国家自然科学基金项目（52077057）；河北省自然科学基金（F2022202023） |
|
| 摘要点击次数: 1486 |
| 全文下载次数: 1699 |
| 中文摘要: |
| 目的 探讨不同参数的重复磁刺激（rMS）对人神经母细胞瘤细胞系SH-SY5Y细胞生长和分化的影响。 方法 将不同频率、不同强度和不同脉冲数的rMS作用于体外培养的SH-SY5Y细胞，按照刺激强度设置最大输出强度的15%组、30%组、60%组，按照刺激频率设置0.5 Hz组、1 Hz组、5 Hz组、10 Hz组、20 Hz组，按照脉冲数设置每日800个脉冲组、1600个脉冲组，所有组均rMS干预4 d。应用四甲基偶氮唑盐比色法(MTT)检测细胞活力。采用神经毒素1-甲基-4-苯基吡啶离子（MPP+）诱导SH-SY5Y细胞凋亡，采用全反式维甲酸诱导SH-SY5Y细胞分化，用免疫组化方法观察神经元特异核蛋白表达及表征细胞分化程度。 结果 在rMS对SH-SY5Y细胞增殖的影响方面，0.5 Hz的rMS抑制SH-SY5Y细胞增殖，10 Hz的rMS促进SH-SY5Y细胞增殖。rMS频率为5 Hz时，最大输出强度15%组和30%组的细胞增殖表现为明显加快（P<0.05）。1600个脉冲组的刺激效果优于800个脉冲组的刺激效果，其中10 Hz的rMS对细胞增殖有明显促进作用（P<0.05）。在rMS对SH-SY5Y细胞凋亡的影响方面，在30%最大输出强度下，0.5 Hz组和10 Hz组细胞凋亡被抑制（P<0.05）。在rMS对SH-SY5Y细胞分化的影响方面，0.5 Hz和10 Hz的rMS均能促进SH-SY5Y细胞向神经元分化。 结论 rMS能影响SH-SY5Y细胞增殖，表现为低频抑制、高频促进，且影响程度会随着刺激脉冲数的增多而提高。rMS对MPP+诱导的SH-SY5Y细胞凋亡具有保护作用，并能促进全反式维甲酸诱导的SH-SY5Y细胞向神经元分化。 |
| 英文摘要: |
| Objective To explore the effect of repeated magnetic stimulation (rMS) on the growth and differentiation of SH-SY5Y human neuroblastoma cells. Methods SH-SY5Y cells were subjected to rMS at 15%, 30% and 60% of the maximum output intensity at frequencies of 0.5Hz, 1Hz, 5Hz, 10Hz and 20Hz. They received either 800 or 1600 pulses per day for 4 days. Cell viability was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Apoptosis was induced using 1-methyl-4-phenylpyridine ion (MPP+) and all-trans retinoic acid was used to induce differentiation. The expression of neuron-specific nuclear proteins and the degree of cell differentiation were observed by immunohistochemistry. Results 0.5Hz rMS inhibited proliferation and 10Hz rMS promoted it. With 5Hz rMS significantly greater cell proliferation was observed at 15% and 30% of the maximum output intensity. The stimulatory effect of 1600 pulses per day was significantly greater than that of 800 pulses, especially at 10Hz. Apoptosis was inhibited at both 0.5Hz and 10Hz with 30% of the maximum output intensity. Meanwhile, both 0.5Hz and 10Hz rMS promoted differentiation of the SH-SY5Y cells into neurons. Conclusions rMS at low frequency inhibits the proliferation of SH-SY5Y cells, but at higher frequency it promotes it. The effect strengthens with more pulses administered. rMS has a protective effect on MPP+-induced SH-SY5Y apoptosis, and it can promote the cells′ differentiation into neurons. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
