徐蕊,汤静,彭朋.运动训练对慢性心力衰竭大鼠循环血液及骨骼肌肾素-血管紧张素系统的影响[J].中华物理医学与康复杂志,2022,44(8):673-678
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| 运动训练对慢性心力衰竭大鼠循环血液及骨骼肌肾素-血管紧张素系统的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2022.08.001 |
| 中文关键词: 运动训练 慢性心力衰竭 大鼠 骨骼肌 肾素-血管紧张素系统 |
| 英文关键词: Exercise Heart failure Skeletal muscles Renin-angiotensin system |
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| 中文摘要: |
| 目的 观察运动训练对慢性心力衰竭（CHF）大鼠循环血液及骨骼肌肾素-血管紧张素系统（RAS）的影响。 方法 采用随机数字表法将50只雄性Wistar大鼠分为安静对照组、运动对照组、模型安静组及模型运动组。将模型安静组及模型运动组大鼠制成CHF动物模型，安静对照组及运动对照组给予假手术处理。运动对照组及模型运动组大鼠均进行8周跑台运动，每周运动5次。于干预8周后采用荧光底物法测定大鼠血浆和比目鱼肌血管紧张素转换酶（ACE）及ACE2活性，采用高效液相色谱法检测血浆和比目鱼肌血管紧张素Ⅱ（AngⅡ）及Ang(1-7)含量，采用Western blot法检测大鼠骨骼肌中AngⅡ 1型受体（AT1R）及Mas受体（MasR）蛋白表达情况。 结果 与安静对照组比较，模型安静组血浆ACE活性升高、ACE2活性下降（P<0.05），模型运动组血浆AngⅡ含量和ACE活性均降低（P<0.05），Ang-(1-7)/AngⅡ比值升高（P<0.05）；与模型安静组比较，模型运动组ACE活性和血浆AngⅡ含量均降低（P<0.05），ACE2活性升高（P<0.05）。与安静对照组比较，模型安静组比目鱼肌AngⅡ含量和AT1R蛋白表达量均升高（P<0.05），模型运动组比目鱼肌MasR蛋白表达量升高（P<0.05）；与模型安静组比较，模型运动组比目鱼肌AngⅡ含量和AT1R蛋白表达量均降低（P<0.05），Ang-(1-7)/AngⅡ比值升高（P<0.05）。 结论 运动训练能诱导CHF大鼠RAS由ACE-AngⅡ-AT1R轴向ACE2-Ang-(1-7)-MasR轴方向转换，并且血浆、骨骼肌中RAS各组分变化特点各异。 |
| 英文摘要: |
| Objective To observe the effect of exercise on the renin-angiotensin system (RAS) in the blood and skeletal muscles of rats with chronic heart failure (CHF). Methods Fifty male Wistar rats were randomly divided into a sedentary control group, an exercise control group, a model sedentary group and a model exercise group. CHF was modelled in the rats of the model sedentary and exercise groups, while those of the sedentary and exercise control groups received a sham operation. The exercise groups performed treadmill exercise for 8 weeks (5 times per week). After the 8 weeks the activity of angiotensin-converting enzyme (ACE) and ACE2 in the plasma and soleus muscle was determined using the fluorescent substrate method. Angiotensin II (AngII) and Ang (1-7) were detected in plasma and the soleus muscle using liquid chromatography. The expression of Ang II type 1 receptor (AT1R) and Mas receptor (MasR) in skeletal muscles was detected using western blotting. Results Compared with the sedentary control group, there was significantly increased average ACE activity and lower average ACE2 activity in the model sedentary group. There was a significant decrease in the average plasma Ang II content and ACE activity, and a significant increase in the Ang-(1-7)/Ang II ratio in the model exercise group. Compared with the model sedentary group, the average ACE activity and plasma Ang II content was significantly lower in the model exercise group but the average ACE2 activity was significantly higher. Compared with the sedentary control group, the average Ang II content and AT1R protein expression in the soleus muscles had increased significantly in the model sedentary group, and the average expression of MasR protein in the soleus muscles increased significantly in the model exercise group. However, compared with the model sedentary group, the average content of Ang II and the average expression of AT1R protein in the soleus muscles had decreased significantly in the model exercise group and the average Ang-(1-7)/Ang II ratio had increased significantly. Conclusion Exercise can induce the conversion of RAS from ACE-Ang II-AT1R to ACE2-Ang-(1-7)-MasR after chronic heart failure, at least in rats. The changes in the components of the RAS in the plasma and skeletal muscle differ. |
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