朱毅,杨雨洁,邵清华,等.电针刺激对脊髓损伤排尿障碍大鼠膀胱c-Kit表达的双向调控作用[J].中华物理医学与康复杂志,2022,44(5):390-394
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| 电针刺激对脊髓损伤排尿障碍大鼠膀胱c-Kit表达的双向调控作用 |
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| DOI：10.3760/cma.j.issn.0254-1424.2022.05.002 |
| 中文关键词: 脊髓损伤 膀胱 电针 |
| 英文关键词: Spinal cord injury Urinary bladder c-Kit Electroacupuncture |
| 基金项目:国家自然科学基金（81403477，81860875） |
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| 摘要点击次数: 1724 |
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| 中文摘要: |
| 目的 观察电针对不同平面脊髓损伤排尿障碍大鼠尿动力学及膀胱c-Kit的调控作用。 方法 将SD雌性大鼠分别制作胸髓和骶髓全横断脊髓损伤模型。于实验第22天将造模成功并进入脊髓损伤后期的大鼠按随机数字表分为胸髓损伤组、胸髓损伤电针组、骶髓损伤组、骶髓损伤电针组，每组取10只，并与10只假手术组对照。2个电针组均取关元、三阴交穴位电针刺激，每日15 min，共14 d。干预结束后，对各组大鼠进行尿动力学检测，采用HE染色观察膀胱形态学改变，采用Real-time qPCR和Western Blotting检测膀胱c-Kit的表达。 结果 与假手术组比较，胸髓损伤组膀胱容量及顺应性均明显降低，残余尿量明显增加（P<0.05），骶髓损伤组的残余尿量、膀胱容量及顺应性均明显增加（P<0.05），且2组膀胱的组织形态也发生了异常改变。胸髓损伤组大鼠的膀胱c-Kit mRNA和蛋白表达均明显升高（P<0.05），骶髓损伤组则明显降低（P<0.05）。分别与2个造模组比较，电针干预增加了胸髓损伤大鼠的膀胱容量和顺应性（P<0.05），电针干预还降低了骶髓损伤大鼠的残余尿量、膀胱容量和顺应性（P<0.05），改善了膀胱的组织形态结构；并使胸髓损伤大鼠膀胱c-Kit mRNA及蛋白表达均明显降低（P<0.05），骶髓损伤大鼠则明显升高（P<0.05）。 结论 电针对膀胱c-Kit的良性双向调控作用，可能是其改善不同平面脊髓损伤尿失禁及尿潴留的效应机制。 |
| 英文摘要: |
| Objective To observe any effects of electroacupuncture (EA) on urodynamics and bladder c-Kit expression in rats with urination disorders after spinal cord injury (SCI). Methods Complete spinal cord injury models were created in female Sprague-Dawley rats by transecting the spine at the thoracic or sacral level. On day 22 after the injury, the rats with successful modeling were randomized into a thoracic spinal cord injury (TSCI) group, a TSCI+EA group, a sacral spinal cord injury (SSCI) group and an SSCI+EA group, each of 10. Both EA groups were given 15 minutes of EA at the Guanyuan (CV4) and Sanyinjiao (SP6) points daily for 14 days. After the intervention, urination function was evaluated using bladder volume, compliance and residual urine volume. Hematoxylin and eosin staining was used to observe any morphological changes in bladder tissues. The gene and protein expression of c-Kit in bladder tissues were detected using real-time quantitative polymerase chain reactions and western blotting. Results Compared with the sham group, the bladder volume and compliance of the TSCI group decreased significantly, while the average residual urine volume increased significantly. In the SSCI group the average residual urine volume, bladder volume and compliance all increased significantly. The modeling altered the morphology of the bladder in all of the SCI rats. The average expression of c-Kit mRNA and protein increased significantly in TSCI group, but both decreased significantly in the SSCI group. EA improved the histological structure of the SCI rats′ bladders. Conclusions EA can bi-directionally regulate bladder c-Kit expression, and that is a possible mechanism for improving urinary incontinence and urine retention after an SCI. |
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