魏居瑞,蒋松涛,刘佩,等.电针调控海马头蛋白mRNA的表达对慢性脑低灌注大鼠学习记忆及海马神经发生的影响[J].中华物理医学与康复杂志,2021,43(9):769-775
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| 电针调控海马头蛋白mRNA的表达对慢性脑低灌注大鼠学习记忆及海马神经发生的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2021.09.001 |
| 中文关键词: 电针 慢性脑低灌注 神经发生 神经干细胞 |
| 英文关键词: Electroacupuncture Cerebral hypoperfusion Neurogenesis Neural stem cells |
| 基金项目:浙江省自然科学基金项目（Y205389,Y2101356)；杭州市临安区科技局项目(临科字2020第31号) |
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| 中文摘要: |
| 目的 通过电针刺激慢性脑低灌注模型大鼠百会穴和大椎穴，观察海马组织头蛋白（Noggin）mRNA的表达，以及电针对慢性脑低灌注模型大鼠学习记忆和海马神经发生的影响。 方法 选取雄性Sprague Dawley(SD)大鼠120只，采用改良永久性结扎双侧颈总动脉法制作慢性脑低灌注模型，将造模成功的104只大鼠分为模型组和电针组，每组52只；再按照治疗时间的不同，将每组大鼠再细分为治疗后第1、2、4、6周四个亚组，每个亚组13只。电针组采用电针刺激大鼠百会穴和大椎穴，电针输出电流1 mA，频率15 Hz连续波，每次20 min，每日1次，治疗7次后休息2 d；模型组不予特殊处理。分别于治疗后第1、2、4、6周，每亚组随机抽取6只大鼠进行BrdU注射，观察神经干细胞增殖及分化情况；利用Morris水迷宫神经行为学检测，观察大鼠空间学习能力和记忆能力的变化情况；采用逆转录-聚合酶链式反应(RT-PCR)检测及BrdU免疫组织化学法，观察海马神经组织Noggin mRNA的表达及海马齿状回神经发生的规律。 结果 电针组大鼠在第2、4、6周的学习记忆能力明显高于同时间点的模型组大鼠（P＜0.05或P＜0.01）；电针组海马组织Noggin mRNA表达均明显高于同时间点模型组大鼠（P＜0.05）；电针组大鼠海马齿状回颗粒下层的BrdU阳性细胞多于同时间点的模型组,且各组的BrdU阳性细胞随着缺血时间的延长均有减少（P＜0.05或P＜0.01）；Pearson相关分析显示，2组大鼠海马Noggin mRNA含量均与BrdU阳性细胞数呈正相关(r=0.561，P=0.000)，且电针组的相关系数大于模型组（P＜0.05）。 结论 电针可通过调控海马Noggin mRNA的表达促进慢性脑低灌注大鼠的海马组织神经发生，从而改善慢性脑低灌注大鼠的空间学习能力和记忆能力。 |
| 英文摘要: |
| Objective To observe the expression of noggin mRNA in the hippocampus of rats with chronic cerebral hypoperfusion, and explore the effect of electroacupuncture (EA) at the "baihui" and "dazhui" acupoints on their learning and memory and on hippocampal neurogenesis. Methods In total, 120 Sprague-Dawley rats had cerebral hypoperfusion induced by permanent bilateral ligation of the common carotid artery. The 104 successfully induced were divided at random into a model group and an EA group, each of 52. The EA group was given EA on the baihui and dazhui acupoints for 20 minutes daily for seven days followed by a two-day break. The output current was 1mA at 15Hz. No special treatment was given to the model group. After one, two, four and six weeks of treatment, 6 rats were given BrdU injections to observe the proliferation and differentiation of neural stem cells. Learning and memory were assessed using the Morris Water Maze. The expression of noggin mRNA and neurogenesis in the dentate gyrus of the hippocampus were measured using reverse-transcription polymerase chain reactions and immunohistochemistry. Results After one, two and five weeks of intervention, the average learning and memory ability of the EA group were significantly better than those of the control group. The average expression of noggin mRNA was significantly higher in the EA group than in the model group at the same time points. Compared with the model group, there were more BrdU-positive cells in the hippocampal dentate gyrus in the EA group, and the number decreased with the prolongation of ischemia. Pearson correlation analysis showed that the levels of noggin mRNA in the hippocampus of both groups were positively correlated with their number of BrdU-positive cells. The correlation was stronger in the EA group than in the model group. Conclusions Electroacupuncture can promote hippocampal neurogenesis in rats with chronic cerebral hypoperfusion by regulating the expression of hippocampal noggin mRNA, thereby improving their spatial learning and memory ability. |
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