王晨宇,寇冠,王治坤,等.有氧运动对实验性结肠炎小鼠氧化应激、炎症反应及细胞凋亡的影响[J].中华物理医学与康复杂志,2021,43(4):301-305
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| 有氧运动对实验性结肠炎小鼠氧化应激、炎症反应及细胞凋亡的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2021.04.003 |
| 中文关键词: 有氧运动 炎症性肠病 氧化应激 炎症反应 细胞凋亡 |
| 英文关键词: Aerobic exercise Inflammatory bowel disease Oxidative stress Inflammatory responses Apoptosis |
| 基金项目:河南省重点科技攻关项目(202102310322) |
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| 中文摘要: |
| 目的 探讨规律有氧运动对实验性结肠炎小鼠氧化应激、炎症反应及细胞凋亡的影响。 方法 采用随机数字表法将45只C57BL/6小鼠分为对照组、模型组及运动组，每组15只。模型组及运动组小鼠均自由饮用3.5%葡聚糖硫酸钠（DSS）溶液进行结肠炎造模。实验进行7 d后对照组及模型组小鼠均置于鼠笼内安静饲养，运动组小鼠则给予8周自主跑轮运动。于末次实验结束48 h后处死各组小鼠，取结肠标本行HE染色并观察结肠组织病理学变化；将余下结肠标本匀浆后测定氧化应激、炎症反应及细胞凋亡情况。 结果 与对照组比较，模型组小鼠结肠组织肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6、前列腺素E2（PGE2）、活性氧（ROS）、丙二醛（MDA）和一氧化氮（NO）含量、诱导型一氧化氮合酶（iNOS）和Caspase-3活性以及环氧合酶2（COX2）、核因子-κB（NF-κB）p65、凋亡诱导因子（AIF）、细胞色素c（Cyt C）、Bax蛋白表达量均显著增加（P<0.05），IL-10、谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化酶（GPx）活性以及Bcl-2蛋白表达量均明显降低（P<0.05）；与模型组比较，运动组TNF-α、IL-1β、IL-6、PGE2、ROS、MDA和NO含量、iNOS和Caspase-3活性以及COX2、NF-κB p65、AIF、Cyt C、Bax蛋白表达量均显著降低（P<0.05），IL-10、GSH含量、SOD和GPx活性以及Bcl-2蛋白表达量均显著升高（P<0.05）。 结论 规律有氧运动能通过改善氧化应激、炎症反应及细胞凋亡等途径对实验性结肠炎小鼠发挥保护作用。 |
| 英文摘要: |
| Objective To explore the effect of regular aerobic exercise on oxidative stress, inflammatory reaction and apoptosis with a rat model of colitis. Methods Forty-five C57BL/6 mice were randomly divided into a control group, a model group and a model exercise group, each of 15. Colitis was induced in the rats of the model and model exercise groups through administration of 3.5% sodium dextran sulfate. The control group and the model group were kept quiet in their cages, while the model exercise group performed voluntary wheel running for 8 weeks after the successful modelling. Any histopathological changes in the colon tissue were observed after hematoxylin and eosin staining. Oxidative stress, inflammatory reaction and apoptosis were measured from colon tissue homogenates. Results Compared with the control group, the average expression of the tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E2 (PGE2) as well as levels of reactive oxygen species (ROS), malondialdehyde (MDA) and nitric oxide (NO) and inducible nitric oxide synthase (iNOS) and Caspase-3 activity and the protein expression of cyclooxygenase 2 (COX2), nuclear factor kappa B (NF-κB) p65, apoptosis inducing factor (AIF), cytochrome c (Cyt C) and Bax had all increased significantly in the other two groups, while the levels of IL-10 and glutathione (GSH), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the protein expression of Bcl-2 protein had decreased significantly in the model group. Compared with the model group, the average content of TNF-α, IL-1 β, IL-6, PGE2, ROS, MDA and NO, the activity of iNOS and Caspase-3 and the protein expression of COX2, NF-κB p65, AIF, CytC and Bax had decreased significantly, while that of IL-10 and GSH, the activity of SOD and GPx and the protein expression of Bcl-2 protein had increased significantly in the model exercise group. Conclusion Regular aerobic exercise has a protective effect against colitis through improving oxidative stress, inflammation and apoptosis, at least in rats. |
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