刘黎黎,董熙远,陈彪.电针刺激三阴交穴对压力性尿失禁大鼠脊髓NMDA受体及α2受体表达的影响[J].中华物理医学与康复杂志,2021,43(3):206-210
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| 电针刺激三阴交穴对压力性尿失禁大鼠脊髓NMDA受体及α2受体表达的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2021.03.003 |
| 中文关键词: 针灸 压力性尿失禁 NMDA受体 α2肾上腺素能受体 |
| 英文关键词: Acupuncture Stress incontinence Urinary incontinence N-methyl-D-aspartic acid receptors α2 adrenergic receptors |
| 基金项目:湖北省卫健委科研基金项目（WJ2019M145） |
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| 中文摘要: |
| 目的 观察电针刺激三阴交穴对压力性尿失禁（SUI）大鼠控尿能力及脊髓N-甲基-D-天冬氨酸(NMDA)受体、α2肾上腺素能受体表达的影响。 方法 采用随机数字表法将48只成年雌性SD大鼠分为3组，其中假手术组大鼠阴道内置入未注水球囊尿管，模型组大鼠行阴道内球囊扩张制作SUI模型，电针组大鼠待阴道内球囊扩张建模成功后，对其三阴交穴给予电针刺激。经1周干预后，3组大鼠均进行尿流动力学检查及腹压漏尿点压（LPP）测定，并同步进行尿道外括约肌肌电描记。另外本研究还分别采用PCR及Western blot方法比较各组大鼠脊髓L6-S1节段NMDA受体及α2受体表达变化。 结果 3组大鼠各项尿流动力学指标组间差异均无统计学意义（P>0.05）。与假手术组比较，模型组大鼠腹压LPP、尿道外括约肌肌电频率及振幅均显著降低（P<0.05）。与模型组比较，电针组大鼠腹压LPP显著提高（P<0.05），尿道外括约肌肌电频率及振幅均显著增加（P<0.05）。与模型组比较，电针组大鼠L6-S1脊髓节段NMDA、α2受体mRNA及蛋白表达均明显上调（P<0.05）。 结论 阴道球囊扩张是制作SUI大鼠模型的有效方法；电针刺激三阴交穴在改善SUI模型大鼠控尿能力同时，还不会影响大鼠正常排尿功能，其治疗机制可能与上调脊髓中NMDA及α2受体水平、增强尿道外括约肌活性有关。 |
| 英文摘要: |
| Objective To explore the effect of electroacupuncture on the expression of N-methyl-D-aspartic acid (NMDA) in the spine and the activity of α2 adrenergic receptors in rats with stress urinary incontinence (SUI). Methods In all, 48 adult female rats were randomly divided into a sham operation group, a model group and an acupuncture group. The sham operation group received sham vaginal distention. Both the model and acupuncture groups had SUI induced using endovaginal balloon dilatation. The former group then had no further intervention, while the latter was given acupuncture on the sanyinjiao point for one week. Urodynamic testing and leak point pressure (LPP) determination were then conducted with all three groups, as well as urethral sphincter electromyography. The expression of NMDA in the L6-S1 spinal segments using the polymerase chain reaction and α2 adrenergic receptor activity was analyzed using western blotting. Results No significant differences in the urodynamic variables were observed among the three groups. However, compared with the sham operation group, a significant decrease in the average LPP and the frequency and amplitude of the external urethral sphincter electromyography was observed in the model group. Moreover, compared with the model group, the acupuncture group showed significantly increased average LPP and higher electromyographic frequency and amplitude. The average mRNA and protein expression of NMDA in the spine were also significantly higher in the acupuncture group, as was the average α2 receptor activity. Conclusions Vaginal distention is an effective method of establishing urinary incontinence in rats. Electroacupuncture can not only improve the ability to store urine, it also increases urination, which may be related to the up-regulation of the expression of NMDA and α2 adrenergic receptors. |
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