吴雨蕊,王红,张若凡,等.重复温热刺激对脂多糖作用后人脐静脉内皮细胞的细胞间黏附分子-1和血管细胞黏附分子-1的影响[J].中华物理医学与康复杂志,2021,43(10):875-879
扫码阅读全文
|
| 重复温热刺激对脂多糖作用后人脐静脉内皮细胞的细胞间黏附分子-1和血管细胞黏附分子-1的影响 |
|
| |
| DOI：10.3760/cma.j.issn.0254-1424.2021.10.003 |
| 中文关键词: 人脐静脉内皮细胞 温热刺激 炎症状态 细胞活力 |
| 英文关键词: Umbilical cells Vein endothelial cells Thermal stimulation Inflammation Cell viability |
| 基金项目:国家自然科学基金资助项目（81973947） |
|
| 摘要点击次数: 2433 |
| 全文下载次数: 2255 |
| 中文摘要: |
| 目的 观察重复温热刺激对炎症状态下人脐静脉内皮细胞（HUVECs）状态及其功能的影响。 方法 将培养好的HUVECs分成空白组、模型组、温热刺激5次组（5次组）、温热刺激9次组（9次组）和温热刺激13次组（13次组），分别接种至五个相同培养条件的培养皿中。空白组为正常HUVECs，不予任何刺激；模型组加入脂多糖（LPS）作为炎症状态模型对照； 5次组、9次组和13次组均先进行重复温热刺激，将恒温箱调至43 ℃，将5次组、9次组和13次组放入其中，持续温热刺激4 min后取出，置于自然环境静置1 min后进行重复温热刺激，3组均接受对应次数的温热刺激。5组HUVECs9（空白组入组后即刻，模型组、5次组、9次组和13次组均于加入LPS后置于37 ℃、5% CO2的恒温细胞培养箱中培养1 h）均采用四甲基偶氮唑盐（MTT）法测定细胞活力，免疫荧光检测核因子-κB（NF-κB）的表达，酶联免疫吸附剂测定ELISA法检测5组HUVECs黏附分子ICAM-1、VCAM-1的水平。 结果 干预后，模型组、5次组和13次组细胞活力的OD值显著低于空白组，差异均有统计学意义（P＜0.05），9次组细胞活力的OD值明显高于模型组、5次组和13次组，差异均有统计学意义（P＜0.05）。干预后，模型组、5次组、9次组和13次组的NF-κB表达量与空白组比较，差异均有统计学意义（P＜0.05）。干预后，9次组NF-κB表达与模型组比较，差异有统计学意义（P＜0.05）。干预后，模型组ICAM-1和VCAM-1的OD值显著增加，5次组、9次组和13次组ICAM-1和VCAM-1的OD值却显著下降，与空白组比较，差异均有统计学意义（P＜0.05）；5次组、9次组、13次组的ICAM-1和VCAM-1的OD值与模型组比较，差异均有统计学意义（P＜0.05）。干预后，9次组ICAM-1的OD值为（43.00±12.96），与5次组的（205.89±10.56）和13次组的（109.87±8.86）比较，差异亦均有统计学意义（P＜0.05）。 结论 重复温热刺激既可以保护炎症状态下HUVECs，也可降低炎症状态下HUVECs的黏附分子的表达。 |
| 英文摘要: |
| Objective To determine any effect of repeated thermal stimulation on the viability and functioning of inflamed human umbilical vein endothelial cells (HUVECs). Methods Well-cultured HUVECs were divided into a normal group, a model group, a thermal stimulation 5 times group (group A), a thermal stimulation 9 times group (group B) and a thermal stimulation 13 times group (group C) and cultured under the same conditions. The normal group was not given any intervention. The model group was stimulated with 1μg/mL lipopolysaccharide for 1 hour. Groups A, B and C were first subjected to 5, 9 and 13 rounds of repeated thermal stimulation, each round lasting 4 minutes at 43℃ and 1 minute at room temperature. They were then incubated for one hour at 37℃ under a 5% CO2 atmosphere with 1μg/mL lipopolysaccharide. Cell viability and the expression of NF-κB were evaluated using methyl thiazolyl tetrazolium and immunofluorescence assays. The levels of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were determined by enzyme-linked immunosorbent assay. Results After the intervention, the average cell viability of the model group and of groups A and C was significantly lower than that of the normal group, while that of group B was significantly higher. After the intervention, the average NF-κB expression in the normal group was significantly different from that in the others, with group B′s level significantly different from that of the model group. After the treatment, the average expression of ICAM-1 and VCAM-1 in the model group had increased significantly, while that in groups A, B and C had decreased significantly compared with the normal group. The levels of groups A, B and C were then significantly different from that of the model group. The average ICAM-1 level of group B was significantly different from those of groups A and C. Conclusions Repeated thermal stimulation can protect inflamed HUVECs and reduce the expression of HUVEC adhesion molecules. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
