贵永玲,李晓勇.有氧运动对衰老大鼠肾脏纤维化的影响[J].中华物理医学与康复杂志,2020,42(9):782-786
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| 有氧运动对衰老大鼠肾脏纤维化的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2020.09.003 |
| 中文关键词: 有氧运动 衰老 肾脏纤维化 TGF-β1/p38MAPK信号途径 上皮-间质转变 |
| 英文关键词: Aerobic exercise Ageing Renal fibrosis TGF-β1/p38MAPK signaling Epithelial-mesenchymal transition |
| 基金项目:河南省科技厅重点科技攻关项目(182102310046) |
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| 中文摘要: |
| 目的 观察规律有氧运动对衰老大鼠肾脏纤维化的影响并探讨其作用机制。 方法 采用随机数字表法将30只雄性SD大鼠分为青年对照组（YC组）、老年对照组（OC组）及老年运动组（OE组），每组10只大鼠。YC组及OC组大鼠均置于鼠笼内安静饲养，OE组大鼠则给予8周中等强度跑台有氧运动。于实验结束后检测各组大鼠24 h尿蛋白、血尿素氮（BUN）及血清肌酐（SCr）含量，采用Masson染色检测肾脏纤维化程度，采用Western Blot技术检测转化生长因子β1（TGF-β1）、p38丝裂原活化蛋白激酶（p38MAPK）、磷酸化p38MAPK（p-p38MAPK）、E-粘钙素及α-平滑肌肌动蛋白（α-SMA）表达水平。 结果 与YC组比较，OC组大鼠24 h尿蛋白、BUN及SCr明显升高（P<0.05），肾脏纤维化程度增加（P<0.05），TGF-β1、p-p38MAPK和α-SMA蛋白表达上调（P<0.05），E-粘钙素表达下调（P<0.05）；与OC组比较，OE组大鼠24 h尿蛋白、BUN及SCr明显降低（P<0.05），肾脏纤维化程度减轻（P<0.05），TGF-β1、p-p38MAPK和α-SMA蛋白表达下调（P<0.05），E-粘钙素表达增加（P<0.05）。 结论 有氧运动能缓解衰老大鼠肾脏纤维化，其作用机制可能与抑制TGF-β1/p38MAPK信号通路及上皮-间质转变有关。 |
| 英文摘要: |
| Objective To explore the effect of regular aerobic exercise on renal fibrosis and its mechanism. Methods Thirty male Sprague-Dawley rats were randomly divided into a young control group (YC group), an old control group (OC) and an old exercise group (OE), each of 10. The YC and OC groups were kept quietly in cages, while the OE group performed moderate-intensity treadmill aerobic exercise for 8 weeks. After the experiment, renal function was evaluated by measuring 24h urine protein, blood urea nitrogen (BUN) and serum creatinine (SCr). The degree of renal fibrosis was detected by Masson staining, and the expressions of transforming growth factor β1 (TGF- β1), p38 mitogen-activated protein kinase (p38MAPK), phosphorylated p38MAPK (p-p38MAPK), E-cadherin, and α-smooth muscle actin (α-SMA) were measured using western blotting. Results Compared with the YC group, a significant increase was observed in the OC group in average 24h urine protein, BUN and SCr, but also in the degree of renal fibrosis, the protein expression of TGF-β1, p-p38MAPK and α-SMA. There was a significant decrease in the average expression of E-cadherin. Compared with the OC group, the average 24h urine protein, BUN and SCr, the degree of renal fibrosis, the expression of TGF-β1, p-p38MAPK and α-SMA protein had all decreased significantly in OE group, while the average expression of E-cadherin had increased significantly. Conclusions Aerobic exercise relieves renal fibrosis in elderly rats, perhaps through inhibiting the TGF-β1/p38MAPK signaling pathway and epithelial-mesenchymal transition. |
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