史昱,黄传,苏悦,等.早期运动干预对脑梗死大鼠皮质脊髓束的影响[J].中华物理医学与康复杂志,2020,42(7):583-587
扫码阅读全文
|
| 早期运动干预对脑梗死大鼠皮质脊髓束的影响 |
|
| |
| DOI：10.3760/cma.j.issn.0254-1424.2020.07.002 |
| 中文关键词: 脑缺血 运动干预 皮质脊髓束 运动功能 |
| 英文关键词: Cerebral ischemia Exercise Corticospinal tract Motor function |
| 基金项目:天津市自然科学基金重点项目（18JCZDJC98900）；天津市卫生局重点发展项目（16KJ122）；“十三五”综合投资及“一流学科”建设项目（11601502-XK0122） |
|
| 摘要点击次数: 3572 |
| 全文下载次数: 4945 |
| 中文摘要: |
| 目的 观察早期运动干预对脑梗死大鼠皮质脊髓束的影响。 方法 取雄性SD大鼠18只，按随机数字表法分为静息组、造模1 d后运动组（1D组）、造模1周后运动组（1W组），每组6只大鼠，3组大鼠均采用改良的Longa线栓法制备大脑中动脉闭塞（MCAO）模型。1D组大鼠在造模1 d后开始运动训练，1W组大鼠在造模1周后开始运动训练，静息组大鼠则每天被置于静止的跑台静息30 min。3组大鼠均于造模1、4、8周后，采用改良神经功能缺损评分法（mNSS）评价的神经功能，核磁共振T2WI序列计算脑梗死体积比，扩散张量成像（DTI）序列检测双侧皮质脊髓束的各向异性分数比值(rFA)，并通过扩散张量纤维束成像（DTT）技术观察皮质脊髓束形态，最后分析rFA值与mNSS评分的相关性。 结果 造模1周后，1D组的mNSS评分显著低于1W组和静息组（P<0.05）；造模4周后，1D组和1W组mNSS评分均显著低于静息组（P<0.05）。造模8周后，1D组mNSS评分显著低于1W组和静息组（P<0.05），且1W组mNSS评分也显著低于静息组（P<0.05）。造模1周和4周后，1D组的梗死体积比显著低于1W组和静息组（P<0.05）。造模4周后， 1W组梗死体积比显著低于静息组（P<0.05）。造模8周后，1D组和1W组的梗死体积比显著低于静息组的0.19±0.03（P<0.05）。造模1周后，1D组的rFA值显著低于静息组（P<0.05）。造模4周后，1D组和1W组的rFA值均显著高于静息组（P<0.05）。造模8周后，1D组rFA值显著高于1W组和静息组，同时1W组的rFA值高于静息的0.49±0.09，差异均有统计学意义（P<0.05）。DTT显示，造模8周后，1D组大鼠的皮质脊髓束比1W组和静息组对称性更好。相关性分析显示，rFA与mNSS评分有较高的相关性（r=-0.707，P=0.001）。 结论 运动干预可以促进脑梗死大鼠皮质脊髓束重塑，改善神经功能，造模1 d后即开始早期运动效果更佳。 |
| 英文摘要: |
| Objective To observe the effect of early exercise intervention on the corticospinal tract of rats with cerebral infarction. Methods Eighteen male Sprague-Dawley rats were randomly divided into a sedentary group (SED), a 1 day later exercise group (1D) and a 1 week later exercise group (1W), each of 6. A modified Longa′s method was used to occlude the middle cerebral artery to model a stroke. Rats in the 1D and 1W groups started exercising 1 day and 1 week after the modeling, while those in the sedentary group were placed on a stationary treadmill for 30 minutes every day. Modified neurological severity scores (mNSSs) were used to quantify neurological functioning after 1, 4 and 8 weeks. Magnetic resonance imaging was used to calculate the infarct volume ratio, and diffusion tensor imaging was used to detect the fractional anisotropy ratio (rFA) of the corticospinal tract for correlation with the mNSS scores. The corticospinal cord′s morphology was observed using DTT. Results After 1 week the average mNSS score of the 1D group was significantly lower than the other two groups′ averages. At 4 weeks the average mNSS scores of both the 1D and the 1W group were significantly lower than the sedentary group′s average. At 8 weeks the 1D group′s average mNSS score was significantly lower than those of the other two groups, while that of the 1W group was significantly lower than the sedentary group′s average. At 1 and 4 weeks after modeling the average infarct volume ratio in the 1D group was significantly lower than those of the other groups. By 4 weeks the average infarct volume ratio of the 1W group was significantly lower than that of the sedentary group, and by 8 weeks the average infarct volume ratios of both the 1D and 1W groups was significantly lower than that of the sedentary group. After 1 week the average rFA of the 1D group was significantly lower than that of the sedentary group, but by 4 weeks the averages of the 1D group and the 1W group were both significantly higher than the sedentary group′s average. At 8 weeks the 1D group′s average rFA was significantly above that of the 1W group and of the sedentary group, and that of the 1W group was significantly higher than that of the sedentary group. After 8 weeks the corticospinal tracts in the 1D group appeared to be more symmetrical than those of the other 2 groups. The rFA results correlated strongly with the mNSS scores (r=-0.707). Conclusions Exercise can promote corticospinal cord remodeling and improve neurological function after cerebral infarction, at least in rats. It should be started as early as possible. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
