胡枫,张平,吴娟,等.窄谱中波紫外线对寻常型银屑病患者Wnt5a/Frizzled2信号分子的影响[J].中华物理医学与康复杂志,2020,42(6):488-492
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| 窄谱中波紫外线对寻常型银屑病患者Wnt5a/Frizzled2信号分子的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2020.06.002 |
| 中文关键词: 寻常型银屑病 窄谱中波紫外线 Wnt5a Frizzled2 |
| 英文关键词: Psoriasis vulgaris Narrow-band ultraviolet B Wnt5a Frizzled2 |
| 基金项目:国家自然科学基金项目（81974474）；湖北省自然科学基金（2018CFC830） |
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| 中文摘要: |
| 目的 观察窄谱中波紫外线（NB-UVB）对寻常型银屑病患者Wnt5a/Frizzled2信号分子表达的影响及其与皮损变化间的相关性，探讨NB-UVB治疗寻常型银屑病的作用机制。 方法 选取32例寻常型银屑病患者及30例体检健康志愿者分别纳入患者组和健康对照组，患者组均采用NB-UVB照射治疗8周。分别采用RT-PCR法、免疫印迹法检测健康对照组及光疗前、后患者组皮肤组织（皮损区、非皮损区）Wnt5a/Frizzled2信号分子mRNA及蛋白表达含量，并统计患者组治疗前、后皮损面积及严重程度指数（PASI）评分，分析Wnt5a/Frizzled2蛋白含量与PASI评分间的相关性。 结果 治疗前患者组皮损区Wnt5a/Frizzled2分子mRNA及蛋白表达均明显高于健康对照组和患者组非皮损区水平，差异均具有统计学意义（P<0.05）；治疗后患者组皮损区Wnt5a/Frizzled2 mRNA及蛋白表达均较治疗前皮损区水平明显下降，差异均具有统计学意义（P<0.05）；治疗前患者组PASI评分为（17.85±6.88）分，皮损区Wnt5a/Frizzled2蛋白表达水平与疾病严重程度间均具有显著正相关性（r=0.743、0.698，P＜0.05）；经NB-UVB治疗后，患者组PASI评分［（4.23±3.11）分］较治疗前明显降低（P<0.05)，此时患者皮损区Wnt5a/Frizzled2蛋白表达水平与疾病严重程度间亦具有显著正相关性（r=0.781、0.723，P<0.01）。 结论 NB-UVB照射可显著下调寻常型银屑病患者皮损区Wnt5a/Frizzled2信号分子mRNA及蛋白表达水平，这可能是NB-UVB照射治疗银屑病患者的重要机制之一。 |
| 英文摘要: |
| Objective To observe the effect of narrow band ultraviolet B (NB-UVB) treatment of patients with psoriasis vulgaris on the expression of Wnt5a and Frizzled2 signaling molecules and its relationship with skin lesions so as to explore the mechanism of treating psoriasis vulgaris with NB-UVB. Methods Thirty-two patients with psoriasis vulgaris and 30 healthy volunteers were selected into a case group and a healthy control group. The case group was treated with NB-UVB irradiation for 8 weeks. The mRNA and protein associated with Wnt5a and Frizzled2 signaling molecules in the skin tissues of both groups were detected before and after the phototherapy using a reverse transcription-polymerase chain reaction and western blotting respectively. Psoriasis area and severity index（PASI） scores were recorded before and after the treatment for the case group, and the correlation between Wnt5a and Frizzled2 protein content and PASI score was analyzed. Results Before the treatment the expression of Wnt5a and Frizzled2 molecular mRNA and protein in the lesioned area of the case group was significantly higher than in the healthy area of the case group and in the healthy group. It had decreased significantly after the treatment. Before the treatment, the expression of Wnt5a and Frizzled2 protein in the lesioned area was significantly and positively correlated with the severity of the disease. However, after the NB-UVB treatment, the average PASI score was significantly decreased. The expression of Wnt5a and Frizzled2 protein in the lesioned area was still significantly and positively correlated with the severity of the disease. Conclusion NB-UVB irradiation can significantly reduce the mRNA and protein expression levels of Wnt5a/Frizzled2 signaling molecules in the lesions of patients with psoriasis vulgaris, which may be one of the mechanisms of NB-UVB in treating psoriasis. |
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