李倩,舒彬,包春茶,等.耐力运动对老年小鼠心肌T-钙粘蛋白的影响及其效应研究[J].中华物理医学与康复杂志,2020,42(6):481-487
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| 耐力运动对老年小鼠心肌T-钙粘蛋白的影响及其效应研究 |
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| DOI：10.3760/cma.j.issn.0254-1424.2020.06.001 |
| 中文关键词: 心肌； 保护； 小鼠； 老年 耐力运动； T-钙粘蛋白 |
| 英文关键词: Myocardium Mice Aging Endurance Exercise T-cadherin |
| 基金项目:国家自然科学基金（31571242） |
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| 摘要点击次数: 3707 |
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| 中文摘要: |
| 目的 观察耐力运动对老年小鼠心肌组织T-钙粘蛋白及其配体脂联素和下游相关分子表达的影响，探讨T-钙粘蛋白的作用。 方法 选取30只17月龄C57雄性小鼠，采用随机数字表法将其分为老年对照组及老年运动组，每组15只；另取15只2月龄C57雄性小鼠纳入为青年对照组。老年运动组小鼠进行12周耐力运动，老年对照组及青年对照组小鼠均正常饲养、无特殊干预。于老年运动组小鼠最后一次运动结束24 h后处死3组小鼠。采用免疫印迹法检测各组小鼠心肌组织T-钙粘蛋白及其配体脂联素表达、腺苷酸活化蛋白激酶(AMPK）磷酸化水平、凋亡及自噬相关信号蛋白B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)、Beclin-1等表达；采用免疫组化法观察各组小鼠心肌T-钙粘蛋白、脂联素及p-mTOR细胞定位和阳性表达。 结果 T-钙粘蛋白定位于心肌细胞胞膜及血管内皮处，脂联素定位于心肌细胞胞膜、胞浆及血管内皮处，p-mTOR定位于心肌细胞胞浆处。与青年对照组比较，老年对照组心肌T-钙粘蛋白、脂联素、AMPK活化水平、抑凋亡蛋白bcl-2、促自噬蛋白Beclin-1表达显著下调（P<0.05），促凋亡蛋白bax、半胱氨酸天冬氨酸蛋白水解酶-9(Caspase-9)表达及自噬相关蛋白mTOR活化水平均显著上调（P<0.05）；与老年对照组比较，老年运动组心肌组织T-钙粘蛋白及配体脂联素、AMPK活化水平、bcl-2、Beclin-1蛋白表达均显著上调（P<0.05)，bax、Caspase-9蛋白表达、mTOR磷酸化水平均显著下调（P<0.05）。 结论 耐力运动可增强老年小鼠心肌T-钙粘蛋白表达，促进脂联素与心肌组织结合，刺激AMPK活化，从而发挥对老龄心肌组织的保护作用。 |
| 英文摘要: |
| Objective To observe the effect of endurance exercise on the expression of T-cadherin, its ligand adiponectin and related downstream molecules in the myocardia of aged mice, and to explore the role of T-cadherin in the observed effects. Methods Thirty 17-month-old C57 male mice were divided randomly into a control group and an exercise group, each of 15. Another 15 2-month-old C57 male mice constituted the young control group. The exercise group performed endurance exercises for 12 weeks, while no exercise was taken in the two control groups. All of the mice were then sacrificed within 24 hours after the exercise group′s last exercise session and the expression of T-cadherin and its ligand adiponectin was measured along with the level of phosphorylation of AMPK, apoptosis and the expression of the autophagy-related signaling proteins bax, bcl-2, Beclin-1 and p-mTOR using western blotting. Immunohistochemistry was used to observe the location and positive expression of T-cadherin, adiponectin and p-mTOR cells in the myocardium. Results The T-cadherin was localized in the membrane and vascular endothelia of cardiomyocytes. Adiponectin was localized in the membrane, cytoplasm and vascular endothelium, and p-mTOR in the cytoplasm. Compared with the young control group, the average T-cadherin, adiponectin, AMPK activation level, apoptotic protein Bcl-2 and autophagy-promoting protein Beclin-1 expression of the aged control group was significantly lower, while the average level of the apoptotic protein bax, Caspase-9 expression, and the phosphorylation of autophagy-related protein mTOR were significantly elevated. Compared with the older control group, the average T-cadherin and ligand adiponectin, AMPK activation level, and Bcl-2 and Beclin-1 protein expression in the exercise group were significantly greater, while the average expression of bax and Caspase-9 protein was significantly lower, as was mTOR phosphorylation. Conclusions Endurance exercise can increase the expression of T-cadherin in the myocardium, promote the binding of adiponectin to myocardial tissue, and stimulate the activation of AMPK, playing a role in protecting the myocardium, at least in elderly mice. |
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