虞记华,艾海波,彭娟,等.电针刺激对膝骨关节炎软骨细胞SIRT1及p53表达的影响[J].中华物理医学与康复杂志,2020,42(2):120-124
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| 电针刺激对膝骨关节炎软骨细胞SIRT1及p53表达的影响 |
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| DOI：DOI:10.3760/cma.j.issn.0254-1424.2020.02.006 |
| 中文关键词: 电针 骨关节炎 软骨 沉默信息调节因子同源蛋白1 |
| 英文关键词: Electro-acupuncture Osteoarthritis Cartilage Silent information regulator 2 homolog 1 |
| 基金项目:四川省卫生厅科研基金资助项目（050075） |
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| 中文摘要: |
| 目的 观察电针刺激对膝骨关节炎（OA）软骨细胞中沉默信息调节因子同源蛋白1 (SIRT1)及p53肿瘤抑制基因表达的影响。 方法 采用随机数字表法将36只实验兔分为对照组、模型组及电针组，每组12只。对照组给予正常饲养，无特殊干预；采用石膏固定法将模型组、电针组实验兔左膝关节伸直位固定6周制成OA模型。制模结束后解除石膏固定，将电针组实验兔绑于治疗台上给予电针刺激，连续治疗16 d；模型组仅固定于治疗台上且不给予电针刺激。待实验结束后按照Mankin评分标准分别对3组实验兔关节软骨肉眼观、光镜下苏木精-伊红（HE）染色结果进行计分；检测各组实验兔软骨细胞SIRT1及p53免疫组化染色灰度值。 结果 电针组关节软骨组织病理学分级、大体形态Mankin评分及HE染色各项Mankin评分均明显优于模型组水平（P<0.05）；3组实验兔软骨细胞中均检测到SIRT1及p53表达；通过进一步比较发现，模型组与对照组、电针组比较，其SIRT1灰度值明显增高（P＜0.01），p53灰度值明显降低（P＜0.01），表明模型组SIRT1表达较电针组、对照组明显降低，p53表达较电针组、对照组明显增高。 结论 电针干预能有效改善膝OA病理表现，可能与上调软骨细胞SIRT1、抑制关节软骨细胞凋亡、促进细胞再生等多重途径有关。 |
| 英文摘要: |
| Objective To investigate any effect of electro-acupuncture stimulation on the expression of the silence regulator homolog 1 (SIRT1) and p53 tumor suppressor genes in chondrocytes of persons with osteoarthritis (OA). Methods Thirty-six experimental rabbits were randomly divided into a control group, a model group and an electro-acupuncture group, each of 12. One knee of each rabbit in the model and electro-acupuncture groups was fixed for 6 weeks to induce OA, while the control group was not given any intervention. The rabbits in the electro-acupuncture group were then treated with electro-acupuncture for 16 days; the model group rabbits were fixed to the treatment table but were not given electro-acupuncture. At the end of the experiment, the articular cartilage was hematoxylin-eosin (HE) stained for microscopic examination and Mankin scoring. The chondrocytes′ gray values after immunohistochemical staining allowed determination of the SIRT1 and p53 expression levels. Results The average histopathological grade, Mankin score and stained Mankin score of the electro-acupuncture group were all superior to the model group′s averages. SIRT1 and p53 expression were detected in all three groups, but the SIRT1 gray value of the model group was significantly higher than the other two groups′ averages. The average p53 gray value in the model group was significantly lower. Conclusions Electro-acupuncture can effectively alleviate the symptoms of knee OA, perhaps by up-regulating SIRT1 expression in the cartilage, inhibiting apoptosis of articular chondrocytes and generally promoting cell regeneration. |
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