江健,熊婧,钟琳阳,等.丘脑电刺激对脑外伤后昏迷大鼠前额叶皮质区去甲肾上腺素α1受体表达水平的影响[J].中华物理医学与康复杂志,2020,42(10):865-869
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| 丘脑电刺激对脑外伤后昏迷大鼠前额叶皮质区去甲肾上腺素α1受体表达水平的影响 |
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| DOI：10.3760/cma.j.issn.0254-1424.2020.10.001 |
| 中文关键词: 丘脑电刺激 脑外伤 昏迷 促醒 去甲肾上腺素α1受体 |
| 英文关键词: Thalamus Brain stimulation Brain trauma Coma α1-adrenergic receptors |
| 基金项目:国家自然科学基金（81660382,81260295） |
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| 中文摘要: |
| 目的 探讨丘脑电刺激对脑外伤后昏迷大鼠的促醒作用及其可能机制。 方法 选取健康成年Wistar种系大鼠45只，随机分为空白对照组、假刺激组和刺激组，每组15只，观察其行为学变化，并通过免疫荧光和Western blot技术检测各组大鼠前额叶皮质组织中去甲肾上腺素α1受体（α1R）的含量。 结果 刺激组中 15 只大鼠全部出现翻正反射，假刺激组仅 8 只大鼠出现翻正反射；将各组大鼠前额叶皮质区α1R含量进行比较，发现刺激组α1R含量水平显著高于假刺激组（P<0.05）。 结论 丘脑电刺激可改善脑外伤后昏迷大鼠的意识状态水平，对促进脑外伤后昏迷大鼠觉醒有积极作用，其机制可能与前额叶皮质去甲肾上腺素α1R表达水平上调有关。 |
| 英文摘要: |
| Objective To investigate the effect of stimulation of the central thalamus (CT-DBS) on awakening from coma after traumatic brain injury and the mechanisms involved. Methods Forty-five healthy, adult Wistar rats were randomly assigned to either a blank control group, a sham stimulation or a stimulation group, each of 15. CT-DBS was applied in the stimulation group. Behavioral changes were observed, and the content of α1-adrenergic receptor (α1R) in the prefrontal cortex (PFC) was measured using immunofluorescence and western blotting. Results The varus reflex was found in all 15 rats in the stimulation group, but in only 8 among the sham stimulation group. The average α1R content in the PFC was significantly higher in the stimulation group than in the sham stimulation group. Conclusions CT-DBS can improve the level of consciousness after traumatic brain injury and promote awakening, at least in rats. The mechanisms may be related to the up-regulation of α1R expression in the PFC. |
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