卫哲,祝力骋.督脉电针对脊髓损伤后MAPK/ERK/1/2信号通路的影响[J].中华物理医学与康复杂志,2020,42(1):8-12
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| 督脉电针对脊髓损伤后MAPK/ERK/1/2信号通路的影响 |
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| DOI：DOI:10.3760/cma.j.issn.0254-1424.2020.01.003 |
| 中文关键词: 脊髓损伤 髓鞘碱性蛋白 细胞外调节蛋白激酶 蛋白激酶B |
| 英文关键词: Spinal cord injury Myelin basic protein Extracellular regulated protein kinases Protein kinase B |
| 基金项目:国家自然科学基金项目(81503639)；浙江省教育厅一般项目(Y201839795)；丽水市科技项目(2017GYX21) |
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| 中文摘要: |
| 目的 观察督脉电针对脊髓损伤（SCI）小鼠MAPK/ERK1/2信号通路相关蛋白表达的影响。 方法 采用随机数字表法将64只C57BL/6小鼠分为假手术组、脊髓损伤组、督脉针刺组和督脉电针组。将脊髓损伤组、督脉针刺组及督脉电针组小鼠制成SCI动物模型。假手术组及脊髓损伤组小鼠制模后均未给予特殊处理，督脉针刺组小鼠于制模后次日给予单纯针刺治疗（取穴大椎、命门），督脉电针组小鼠于制模后次日给予电针治疗，取穴方法同督脉针刺组，电刺激设置疏密波（2/100 Hz），电刺激强度0.2 mA。于制模后3 d、7 d、14 d及28 d时分别采用免疫荧光、蛋白印迹法测定各组小鼠受损脊髓p-ERK1/2、p-Akt及髓鞘碱性蛋白(MBP)表达情况。 结果 与脊髓损伤组同期比较，督脉电针组在制模后3 d、14 d及28 d时，督脉针刺组在制模后28 d时其受损脊髓p-ERK1/2蛋白表达均显著增强(P<0.05)；与督脉针刺组比较，督脉电针组在制模后3 d、14 d时其受损脊髓p-ERK1/2蛋白表达均明显增强(P<0.05)。督脉电针组在制模后14 d、28 d时，督脉针刺组在制模后28 d 时其受损脊髓p-Akt蛋白表达均较脊髓损伤组显著增强(P<0.05)，督脉针刺组在制模后3 d时其受损脊髓p-Akt蛋白表达较脊髓损伤组明显减弱(P<0.05)。督脉电针组在制模后3 d、7 d、14 d时，督脉针刺组在制模后3 d、7 d时其受损脊髓MBP蛋白表达均较脊髓损伤组明显增强(P<0.05)。 结论 督脉电针可促进SCI小鼠p-ERK1/2、p-Akt及MBP蛋白表达。 |
| 英文摘要: |
| Objective To observe the effect of applying electro-acupuncture to the governor vessel on the expression of proteins in the MAPK/ERK1/2 signaling pathway. Methods Sixty-four female C57BL/6 mice were randomly divided into a sham group, a spinal cord injured (SCI) group, an acupuncture (AP) group and an electro-acupuncture (EA) group. An SCI model was induced in all of the rats except those in the sham group. The sham and SCI groups were not given any special treatment, while the AP and EA groups were treated with conventional or electro-acupuncture applied to the Dazhui (DU14) and Mingmen (DU4) acupoints in the governor vessel beginning on the day following the operation. The electrical stimulation was in dense-disperse waves at 2/100Hz frequency and 0.2mA intensity，lasted for 15 minutes daily, 5 days a week for 4 weeks . The expression of myelin basic protein (MBP), the phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and the phosphorylation of protein kinase B (Akt) in the injured volume were determined using immunofluorescence and western blotting on the 3rd, 7th, 14th and 28th days after the modeling. Results Compared with the SCI group, p-ERK1/2 expression was greatly enhanced in the EA group on the 3rd, 14th and 28th days. It was also significantly enhanced in the AP group by the 28th day. Compared with the AP group, the average p-ERK1/2 expression in the EA group was significantly enhanced on the 3rd and 14th days. Compared with the SCI group, the average p-Akt of the EA group was significantly enhanced on the 14th and 28th days. The average p-Akt of the AP group had decreased significantly on the 3rd day. Compared with the SCI group, the average expression of MBP in the EA group was enhanced significantly on the 3rd, 7th and 14th days and the AP group′s average was significantly greater on the 3rd and 7th days. Conclusion Electro-acupuncture can promote the expression of MBP, the phosphorylation of ERK1/2 and the phosphorylation of Akt after spinal cord injury. |
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