吴福春,陈捷,余德标,等.温针灸对兔膝骨关节炎软骨细胞及Lubricin的影响[J].中华物理医学与康复杂志,2019,41(8):565-569
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| 温针灸对兔膝骨关节炎软骨细胞及Lubricin的影响 |
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| DOI：DOI:10.3760/cma.j.issn.0254-1424.2019.08.002 |
| 中文关键词: 温针灸 Lubricin蛋白 膝骨关节炎 细胞凋亡 |
| 英文关键词: Needle-warmed moxibustion Lubricin protein Knee osteoarthritis Apoptosis |
| 基金项目:福建省立医院优秀青年医师科研项目（2014071）;福建医科大学启航科研项目（2017XQ1125） |
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| 中文摘要: |
| 目的 探讨温针灸对膝骨关节炎（KOA）实验兔软骨细胞凋亡及关节润滑素（Lubricin）蛋白含量的影响，为温针灸治疗KOA提供理论支持。 方法 采用随机数字表法将40只新西兰大白兔分为假手术组、模型组、西药组及温针灸组，每组10只。通过注射木瓜蛋白酶水溶液将模型组、西药组及温针灸组实验兔制成兔KOA模型，假手术组仅将注射针头刺入髌骨下极髌腱外缘后撤出。温针灸组于制模后给予“内犊鼻”、“外犊鼻”温针灸治疗，治疗14 d为1个疗程，共治疗2个疗程；西药组于制模后给予双氯芬酸钠（按15 mg/kg体重）灌胃，疗程同温针灸组；假手术组及模型组均正常饲养，不给予特殊处理。于疗程结束后，取各组实验兔膝关节软骨组织进行相关检测，比较各组实验兔膝关节软骨细胞数量及软骨细胞Lubricin表达情况。 结果 与模型组及西药组比较，温针灸组软骨细胞数量明显增多，且细胞排列整齐；TUNEL染色显示温针灸组软骨细胞凋亡率明显降低（P<0.05）；RT-PCR检测显示温针灸组Lubricin mRNA表达明显增强（P<0.05）；Western blot检测显示温针灸组Lubricin蛋白表达明显增强（P<0.05）。 结论 温针灸对KOA实验兔具有积极治疗作用，能抑制膝关节软骨细胞凋亡，促进Lubricin分泌，提示温针灸治疗KOA可能与减少软骨细胞凋亡及增强Lubricin表达有关。 |
| 英文摘要: |
| Objective To investigate the effect of needle-warmed moxibustion on chondrocyte apoptosis and the lubricin protein content of osteoarthritic knees. Methods Forty New Zealand white rabbits were randomly divided into a sham operation group, a model group, a Western medicine group and a needle-warmed moxibustion group, each of 10. Knee osteoarthritis was induced in all except the sham operation group by injecting an aqueous solution of papain into the outer edge of one patellar tendon at the inferior pole of the patella. In the sham operation group the injection needle was only inserted and withdrawn. The needle-warmed moxibustion group had needle-warmed moxibustion applied to the internal and external DUBI acupoints 14 days after the modelling in 2 courses of treatment. The Western medicine group was given diclofenac sodium (15 mg/kg body weight) by gavage on the same schedule. The sham operation group and the model group were fed normally without special treatment. At the end of the treatment, knee cartilage tissue was resected from each group and the number of chondrocytes and the expression of lubricin in the articular cartilage cells were compared. Results Compared with the model group and the Western medicine group, the number of chondrocytes in the moxibustion group was significantly greater and the cells were arranged more neatly. TUNEL staining showed that the apoptosis rate of chondrocytes in the moxibustion group was significantly lower. A reverse transcription polymerase chain reaction showed that the expression of lubricin mRNA in the moxibustion group had increased significantly. Western blotting showed that the expression of lubricin protein was also significantly enhanced in the moxibustion group. Conclusions Needle-warmed moxibustion has a positive therapeutic effect on knee osteoarthritis, at least in rabbits. It can inhibit the apoptosis of knee chondrocytes and promote the secretion of lubricin. |
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