刘玲,陈祢,王宝兰.超短波对间充质干细胞粘附增殖及成骨能力的影响[J].中华物理医学与康复杂志,2019,41(4):241-245
扫码阅读全文
|
| 超短波对间充质干细胞粘附增殖及成骨能力的影响 |
|
| |
| DOI：DOI:10.3760/cma.j.issn.0254-1424.2019.04.001 |
| 中文关键词: 超短波 增殖 粘附 骨髓间充质干细胞 成骨分化 |
| 英文关键词: Ultrashortwave irradiation Proliferation Cell adhesion Mesenchymal stem cells Osteogenic differentiation |
| 基金项目:新疆维吾尔自治区自然科学基金面上项目（2015211C067） |
|
| 摘要点击次数: 3691 |
| 全文下载次数: 3817 |
| 中文摘要: |
| 目的 观察鼠骨髓间充质干细胞（rMSCs）受超短波作用后其粘附、增殖及成骨分化能力的变化。 方法 将rMSCs置于超短波理疗仪上，以每日1次、每次15 min的条件进行处理，1 d后检测细胞早期粘附能力，2 d后借助倒置荧光显微镜观察细胞形态，1 d、3 d、7 d检测细胞增殖能力，7 d后检测成骨相关基因骨桥蛋白(OPN)表达量。 结果 与未经超短波处理的对照组相比，接受超短波处理的超短波组早期细胞粘附数量为前者的1.8倍（P<0.05）；2 d后超短波组比对照组细胞的伪足数量多，伪足铺展范围大；超短波组细胞连续培养7 d后，与对照组相比，其增殖能力提升（P<0.05），OPN表达上调（P<0.05）。 结论 超短波能影响rMSCs的早期粘附能力和形态，能促进其增殖和向成骨方向分化。 |
| 英文摘要: |
| Objective To observe the effect of ultrashortwave irradiation on the adhesion, proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (rMSCs) in rats. Methods The rMSCs were put under ultrashortwave radiation for 15 minutes every day. Early cell adhesion was detected after 1 day of the treatment. The cell morphology was observed under an inverted fluorescence microscope on the 3rd day, the cell proliferation was measured on the 1st, 3rd and 7th day, while the expression of the osteogenic gene osteopontin was detected on the 8th day. Results Early cell adhesion increased 80% (a significant increase) and more pseudo-feet with a larger range of spread were observed. After 7 days of irradiation the proliferation and the expression of osteogenic gene osteopontin had both increased significantly. Conclusion Ultrashortwave irradiation can affect the early adhesion and morphology of rMSCs and promote their proliferation and osteogenic differentiation. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
