刘伟军,王威,黎清波,等.脉冲电磁场对人退变髓核细胞A2A腺苷受体的影响[J].中华物理医学与康复杂志,2019,41(11):818-822
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| 脉冲电磁场对人退变髓核细胞A2A腺苷受体的影响 |
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| DOI：DOI:10.3760/cma.j.issn.0254-1424.2019.11.004 |
| 中文关键词: 脉冲电磁场 腺苷受体 髓核细胞 炎症因子 |
| 英文关键词: Pulsed electromagnetic fields Adenosine receptors Nucleus pulposus cells Inflammatory cytokines |
| 基金项目:湖北省自然科学基金面上项目（2018CFB720） |
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| 中文摘要: |
| 目的 观察脉冲电磁场对人退变髓核细胞A2A腺苷受体及白细胞介素1（IL-1）、肿瘤坏死因子-α（TNF-α）表达的影响。 方法 取人退变髓核细胞进行体外培养，取生长良好的二代髓核细胞给予脉冲电磁场干预（磁场强度0.8 mT，频率50 Hz，脉宽150 μs，曝磁时间30 min，共曝磁6次）。采用蛋白免疫印迹法（WB）及RT-PCR法观察髓核细胞A2A腺苷受体表达变化，采用ELISA法检测髓核细胞炎症因子IL-1β、TNF-α表达变化。另外本研究还分别采用A2A腺苷受体拮抗剂和激动剂处理人退变髓核细胞，再采用ELISA法检测脉冲电磁场对人退变髓核细胞IL-1β、TNF-α表达的影响。 结果 人退变髓核细胞经脉冲电磁场处理后，发现A2A腺苷受体表达水平明显增强（P<0.05），同时IL-1β、TNF-α表达水平明显降低（P<0.05）。人退变髓核细胞经A2A腺苷受体拮抗剂处理后，发现能逆转脉冲电磁场对IL-1β、TNF-α的抑表达作用；人退变髓核细胞经A2A腺苷受体激动剂处理后，发现能增强脉冲电磁场对IL-1β、TNF-α的抑表达作用。 结论 脉冲电磁场干预能明显抑制人退变髓核细胞炎症因子IL-1β、TNF-α表达，并且该抑制作用可能与上调A2A腺苷受体表达有关。 |
| 英文摘要: |
| Objective To explore the expression of the A2A adenosine receptor and the inflammatory cytokines interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in human degenerative nucleus pulposus (NP) cells after they have been treated with a pulsed electromagnetic field (PEMF). Methods Human degenerative NP cells were cultured in vitro and treated using an 0.8mT PEMF with a pulse frequency of 50Hz. The pulse width was 150μs and the exposure time was 30min, repeated 5 times at 12 hour intervals. The expression of the A2A adenosine receptor in NP cells was determined using western blotting and reverse transcription polymerase chain reactions. The expression of the inflammatory cytokines IL-1β and TNF-α were detected using enzyme-linked immunosorbent assays (ELISA). The human degenerative NP cells were also treated with an antagonist and agonist of the A2A adenosine receptor, and the expression of IL-1β and TNF-α were also determined using ELISA. Results After the PEMF treatment the expression of the A2A adenosine receptor increased significantly, while the expression of IL-1β and TNF-α decreased significantly. However, the A2A adenosine receptor antagonist reversed the inhibitory effect of the PEMF on the expression of IL-1β and TNF-α, while the agonist played an opposite role. Conclusion A PEMF can significantly inhibit the expression of IL-1β and TNF-α in human degenerative NP cells, which could be related to up-regulation of the expression of the A2A adenosine receptor in those cells. |
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