苏浩,杜姝,陈丹莹,等.早期跑台训练联合超短波治疗对脊髓损伤大鼠功能恢复的影响[J].中华物理医学与康复杂志,2019,41(10):721-726
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| 早期跑台训练联合超短波治疗对脊髓损伤大鼠功能恢复的影响 |
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| DOI：DOI:10.3760/cma.j.issn.0254-1424.2019.10.001 |
| 中文关键词: 脊髓损伤 跑台训练 超短波 水通道蛋白-4 胶质纤维酸性蛋白 |
| 英文关键词: Spinal cord injury Treadmill training Ultrashort wave irradiation Auaporin protein-4 Glial fibrillary acidic protein |
| 基金项目:国家自然科学基金青年科学基金项目(81101462)；辽宁省自然科学基金项目（201602875）；辽宁省科学事业公益研究基金项目（2016003001） |
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| 中文摘要: |
| 目的 观察早期跑台训练联合超短波治疗对大鼠脊髓损伤（SCI）后4周BBB评分、损伤部位水通道蛋白-4（AQP-4）和胶质纤维酸性蛋白(GFAP)表达的影响，探讨两种方法单独应用及联合应用对SCI的疗效及作用机制。 方法 选取50只雌性SD大鼠，采用改良Allen′s法制作大鼠SCI模型，造模成功（40只）后按照随机数字表法将其分为假手术组、对照组、超短波组、跑台组和联合组，每组8只。术后4周，用BBB评分法评价大鼠后肢运动功能的恢复情况。术后4周取材，SCI部位行GFAP和AQP-4免疫组化染色，测定蛋白表达的积分光密度值（IOD），进行比较分析。 结果 假手术组BBB评分为21分。余4组术后1 d的BBB评分为0～1分，随着时间延长，评分逐渐增加，除假手术组外，余4组术后4周的BBB评分显著增高（P<0.05）。与对照组同时间点比较，跑台组、联合组大鼠的BBB评分显著较高（P<0.05）。与超短波组同时间点比较，跑台组术后4周［（12.88±3.04）分］，联合组术后2周［（10.12±1.13）分］、3周［（12.38±1.19）分］及4周［（14.50±1.31）分］的BBB评分较高（P<0.05）。SCI术后4周，超短波组、跑台组及联合组AQP-4 IOD均较对照组低（P<0.05），联合组AQP-4 IOD较超短波组低（P<0.05）。SCI后4周，超短波组、跑台组、联合组GFAP IOD均较对照组低（P<0.05）。与超短波组比较，联合组GFAP IOD较低（P<0.05）。与跑台组比较，联合组GFAP较低（P<0.05）。 结论 跑台训练、超短波治疗均对大鼠SCI后的运动功能恢复有积极作用，其治疗机制可能与减轻损伤部位的AQP-4和GFAP表达有关，且联合应用的疗效更为优异。 |
| 英文摘要: |
| Objective To investigate the effect of early-stage training combined with the ultrashort wave therapy on the functional recovery of rats after a spinal cord injury, and to observe the expression of aquaporin protein-4 (AQP-4) and glial fibrillary acidic protein (GFAP). Methods Fifty female Sprague-Dawley rats had spinal cord injury (SCI) induced using the modified Allen′s method. After successful modeling, 40 were randomly divided into a sham operation group, a control group, an ultrashort wave group, a treadmill group and a combined group, each of 8. Motor function in their hind limbs was evaluated 4 weeks after the operation using BBB scoring. GFAP and AQP-4 immunohistochemical staining were used to determine the integral optical density (IOD) of the protein expression. Results The average BBB score of the sham operation group was 21, while the other four groups averages were all less than 1 on the 1st day after the operation. They gradually increased with time, and by 4 weeks the increases were significant. Compared with the control group at the same time point, the average BBB scores of the treadmill and the combined groups were significantly higher. Compared with the ultrashort wave group, the average BBB score of the treadmill group was higher after 4 weeks, and the combined group′s average was significantly higher at 2, 3 and 4 weeks after the operation. Four weeks after the SCI modeling, the average AQP-4 IOD and GFAP IOD levels of the ultrashort wave group, the treadmill group and the combined group were lower than that of the control group, while the average AQP-4 and GFAP IOD levels of the combined group were significantly lower than those of the ultrashort wave group. Compared with the treadmill group, the combined group had a significantly lower average GFAP level. Conclusions Both treadmill training and ultrashort wave treatment promote motor function recovery after a spinal cord injury. The mechanism may be related to downregulation of AQP-4 and GFAP expression at the injured site. Combining the two treatments gives better effects. |
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