张逸仙，刘楠，袁明洲，郑谋炜，杜厚伟，陈荣华.跑台运动训练对脑缺血大鼠TGF-β1/Smad3通路蛋白表达及细胞凋亡的影响[J].中华物理医学与康复杂志,2018,40(8):564-568
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| 跑台运动训练对脑缺血大鼠TGF-β1/Smad3通路蛋白表达及细胞凋亡的影响 |
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| DOI： |
| 中文关键词: 脑缺血 运动训练 转化生长因子 凋亡 |
| 英文关键词: Cerebral ischemia Exercise Transforming growth factor Smad3 protein Apoptosis |
| 基金项目:国家自然科学基金面上项目(81472161)，福建省自然科学基金面上项目(2018J01309)，第二届福建省科技创新领军人才项目(2016B014) |
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| 中文摘要: |
| 目的 观察跑台运动训练对脑缺血大鼠脑缺血周边区组织中转化生长因子-β1（TGF-β1）及其受体Smad3蛋白表达水平及细胞凋亡的影响，探讨跑台运动训练促进大鼠脑缺血后神经功能恢复的相关机制。 方法 取成年雄性Sprague-Dawley(SD)大鼠30只，按随机数字表法分为假手术组（n=6）、模型组（n=12）和运动组（n=12）。模型组和运动组大鼠采用改良的Longa线栓法制备大脑中动脉闭塞(MCAO)脑缺血动物模型，假手术组大鼠手术方法同模型组和运动组，但是不插入线栓。运动组于造模成功后24 h采用跑台训练仪进行跑台运动训练，其余2组则不进行运动训练。采用修正的神经功能缺损评分方法(mNSS)评价模型组和运动组大鼠造模后第3天、7天及14天的神经功能，并于造模后14 d断头取脑。采用Western blot法检测脑缺血周边区组织中TGF-β1及Smad3蛋白的表达情况，采用TUNEL法检测脑缺血周边区组织中细胞凋亡情况。 结果 造模后第7、14天，运动组的mNSS评分分别为(7.06±0.79)分和(6.33±1.15)分，分别于模型组同时间点比较，差异均有统计学意义(P<0.05或P<0.01)；运动组大鼠的TGF-β1及Smad3蛋白表达均较模型组明显增加，差异具有统计学意义(P<0.01)；运动组大鼠脑缺血周边区TUNEL染色阳性细胞较模型组明显减少，差异具有统计学意义(P<0.01)。 结论 跑台运动训练能够促进脑缺血后大鼠神经功能恢复，其机制可能与上调脑缺血周边区组织中TGF-β1/Smad3蛋白表达，进而抑制细胞凋亡有关。 |
| 英文摘要: |
| Objective To observe the effect of treadmill exercise on the expression of transforming growth factor-β1 (TGF-β1) and its receptor Smad3 protein as well as on cell apoptosis in the ischemic boundary zone, so as to explore how exercise promotes the recovery of neurological function after cerebral ischemia. Methods Thirty adult male Sprague-Dawley rats were randomly divided into a sham group (n=6), a model group (n=12) and an exercise group (n=12). A modified Longa′s method was used to establish an animal model of cerebral ischemia by occluding the right middle cerebral artery in the rats of the model and exercise groups. Those of the sham group were subjected to the same surgical procedure except that no thread was inserted. After 24h the exercise group began treadmill training, while the other two groups were left on the treadmill without training. Modified neurological severity scores (mNSSs) were used to quantify the rats′ neurological functioning on the 3rd, 7th and 14th day after the surgery. The ischemic boundary zone tissue was then dissected to detect the expression of TGF-β1 and Smad3 protein using western blotting. Cell apoptosis was detected by TUNEL staining. Results The average mNSS scores of the exercise group on the 7th and the 14th day were significantly lower than those of the model group at the same time points. The average expression level of TGF-β1 and Smad3 protein in the exercise group was significantly higher than in the model group. The number of TUNEL-positive cells in the exercise group was significantly lower than in the model group on the 14th day. Conclusions Treadmill exercise can improve the recovery of neurological function after cerebral ischemia. It may be partly due to upregulating the expression of TGF-β1 and Smad3 protein, which inhibit cell apoptosis in the ischemic boundary zone. |
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