张超，王平，刘爱峰，张君涛，尹广斌，金哲峰，李远栋，杨硕，王晓东.双极射频控温热凝器体外量效关系的实验研究[J].中华物理医学与康复杂志,2018,40(6):414-417
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| 双极射频控温热凝器体外量效关系的实验研究 |
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| DOI： |
| 中文关键词: 双极射频 蛋清实验 剂量效应关系 |
| 英文关键词: Radio frequency heating Dose-effect relationships Egg white Denaturation |
| 基金项目:国家自然科学基金委员会资助面上项目资助(81673994，51573137) |
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| 中文摘要: |
| 目的 研究双极射频控温器时间、预设温度、间距对两电极热效应的影响。 方法 选择新鲜鸡蛋清，按随机数字表法分为不同间距组、温度组和时间组，固定2个射频电极保持针身垂直置入方盘中央蛋清内，用预设温度（50～90℃）、射频时间（60s、120s）、电极间距（0.25、0.50、1.00和2.00cm）进行干预，通过游标卡尺、秒表、阻抗及温度检测，观测两电极真实温度、出现纺锤形连接的间距、不同温度下蛋清变性的时间跨度、蛋清阻抗变化、蛋清变性的最低温度、不同时间蛋清变性量（体积）和不同温度下蛋清变性阻抗的变化。 结果 ①当电极间距固定保持2.00cm，预设温度设定在63℃，主电极真实温度为63.7℃，次电极真实温度52.6℃，次极温度与预设温度、主极温度的分布差异有统计学意义（P<0.05），电极间距在0.25、0.50、1.00cm时，次极温度-预设温度和主极温度-次极温度差异有统计学意义（P<0.01）；②在间距为0.25cm，56℃时开始出现蛋清变性，在0.25cm、0.50cm间距，温度70℃以上两电极之间出现纺锤形连接，在1.00cm和2.00cm间距时同样温度未出现该现象；③在间距为0.25cm时，预设温度75℃，单次射频60s、单次射频120s及2个单次射频60s后蛋清体积分别为(90.21±0.64)mm3、(95.08±0.53)mm3、(92.88±0.74)mm3，蛋清的变性体积差异均有统计学意义(P<0.01)；④单次射频60s蛋清射频前、后电阻差异无统计学意义（P=0.11），单次射频120s蛋清射频前和射频后电阻分别为（128.41±8.04）Ω和（121.29±8.04）Ω，差异有统计学意义（P=0.02）。 结论 双极射频能使蛋清组织变性，温度越高、时间越长、电极间距越小引起的变性效率越高。 |
| 英文摘要: |
| Objective To study the effect of the preset temperature, heating time and the spacing of radio frequency electrodes. Methods Fresh egg white was used to study the effects of heater spacing, temperature and heating time. Two electrodes were fixed to keep the needles perpendicular to the center of egg white confined on a square plate. Temperatures from 50 to 90℃ were tested with heating times of 60 and 120 seconds and electrode spacings of 2.0, 1.0, 0.5 and 0.25 centimetres. The real-time temperature and the time to the appearance of a spindle-shaped bridge between the electrodes was observed. The egg white′s impedance and resistance, the lowest temperature of denaturation, and the mass (or volume) denatured at different time points were also observed. Results With an electrode spacing of 2 cm and the thermostat set at 63℃, the true electrode temperatures were 63.7 and 52.6℃, with the secondary electrode the cooler. Significant differences between the secondary electrode temperature and the preset temperature as well as the temperature between the primary and the secondary electrodes were observed. It was also found that different electrode spacing caused significant differences between the preset temperature and the primary as well as the secondary electrode temperature . Denaturation began at 56℃ with a spacing of 0.25 cm. At spacings of 0.25 and 0.50 cm, a spindle-shaped connection between the two electrodes was observed at temperatures above 70℃, but higher temperature was required at 1.00 and 2.00 cm. When the spacing was 0.25 cm and the preset temperature was 75℃, the denatured volume after 60 s of heating was 90.21±0.64 mm3. Heating for 120 s denatured 95.08±0.53 mm3 and two 60 s heatings 92.88±0.74 mm3, all significant differences. There were no significant differences in the resistance of the egg white before and after a single heating for 60 s, but after a single heating lasting 120 s it had increased significantly from 128.41±8.04 to 121.29±8.04 Ω. Conclusion Bipolar radio frequency heating can denature egg white. Higher temperature, longer heating and smaller electrode spacing heat more effectively. |
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